Antibody data
- Antibody Data
- Antigen structure
- References [2]
- Comments [0]
- Validations
- Western blot [5]
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Validation data
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- Product number
- NBP1-76465 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NBP1-76465, RRID:AB_11024048
- Product name
- Rabbit Polyclonal MyD88 Antibody
- Antibody type
- Polyclonal
- Description
- Peptide affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 mg
- Concentration
- 1 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Submitted references Disruption of downstream MyD88 or TRIF Toll-like receptor signaling does not protect against cerebral ischemia.
Contribution of TLR2 to the initiation of ganglioside-triggered inflammatory signaling.
Famakin BM, Mou Y, Ruetzler CA, Bembry J, Maric D, Hallenbeck JM
Brain research 2011 May 4;1388:148-56
Brain research 2011 May 4;1388:148-56
Contribution of TLR2 to the initiation of ganglioside-triggered inflammatory signaling.
Yoon HJ, Jeon SB, Suk K, Choi DK, Hong YJ, Park EJ
Molecules and cells 2008 Feb 29;25(1):99-104
Molecules and cells 2008 Feb 29;25(1):99-104
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MyD88 Antibody [NBP1-76465] - Analysis of MYD88 in Daudi cell lysate with MYD88 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MyD88 Antibody [NBP1-76465] - Validation with MyD88 siRNA Knockdown: HeLa cells were transfected with control siRNAs (lane 1) or MyD88 siRNAs (lane 2). Loading: 10 ug of HeLa whole cell lysates per lane. Antibodies: NBP1-76465 (2 ug /mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MyD88 Antibody [NBP1-76465] - Animal Species Reactivity Loading: Lysates/proteins at 15 ug per lane. Antibodies: NBP1-76925 (2 ug/mL) or NBP1-76465 (2 μg/mL). 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Cell Lines Loading: 15 ug of lysates per lane. Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MyD88 Antibody [NBP1-76465] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human Tissues Loading: 15 ug of lysates per lane. Antibodies: MyD88 NBP1-76925 (2 ug/mL), MyD88 NBP1-76465 (2 ug/mL), beta-actin (1 ug/mL), and GAPDH (0.02 ug/mL), 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate.