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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-84846 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XIAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Heat-mediated antigen retrieval is recommended for the staining of paraffin sections. Rabbit anti Human XIAP antibody recognizes human X-chromosome linked inhibitor of apoptosis (XIAP).
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µg
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references Antibody-drug nanoparticle induces synergistic treatment efficacies in HER2 positive breast cancer cells.
Disruption of XIAP-RIP2 Association Blocks NOD2-Mediated Inflammatory Signaling.
Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-Hep1 cells in vitro.
Abedin MR, Powers K, Aiardo R, Barua D, Barua S
Scientific reports 2021 Apr 1;11(1):7347
Scientific reports 2021 Apr 1;11(1):7347
Disruption of XIAP-RIP2 Association Blocks NOD2-Mediated Inflammatory Signaling.
Goncharov T, Hedayati S, Mulvihill MM, Izrael-Tomasevic A, Zobel K, Jeet S, Fedorova AV, Eidenschenk C, deVoss J, Yu K, Shaw AS, Kirkpatrick DS, Fairbrother WJ, Deshayes K, Vucic D
Molecular cell 2018 Feb 15;69(4):551-565.e7
Molecular cell 2018 Feb 15;69(4):551-565.e7
Amentoflavone enhances sorafenib-induced apoptosis through extrinsic and intrinsic pathways in sorafenib-resistant hepatocellular carcinoma SK-Hep1 cells in vitro.
Chen WL, Hsieh CL, Chen JH, Huang CS, Chen WT, Kuo YC, Chen CY, Hsu FT
Oncology letters 2017 Sep;14(3):3229-3234
Oncology letters 2017 Sep;14(3):3229-3234
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of human kidney lysate probed with a XIAP polyclonal antibody (Product # PA1-84846) at 0.5 (A), 1 (B) and 2 (C) µg/mL
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical staining of human formalin fixed paraffin processed kidney tissue using a XIAP polyclonal antibody (Product # PA1-84846)
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Western blot analysis: The cell signaling protein expressions of ( a ) BT-474 and ( b ) MDA-MB-231 cells using PBS, PTX alone, TTZ alone, and PTXNR-TTX treatments. The relative intensity of XIAP, actin, cleaved caspase-9, caspase 3, cleaved caspase 3, and cytochrome C is analyzed after 48 h of treatment in ( c ) BT-474 and ( d ) MDA-MB-231 cells using ImageJ/ Fiji. For each protein expression the experiment was replicated at least n = 2-4 times and the average data is presented as mean +- standard deviation. The relative protein expressions are normalized to the untreated control cells and to the housekeeping GAPDH protein expression (hypothesized expression mean, u = 1). The p values for overexpression or downregulation of cytochrome-C, cleaved caspase-3, and XIAP in BT-474 are 0.039, 0.003, and 0.000049, respectively. The p value for the overexpression of cytochrome-C in MDA-MB-231 is 0.047. ***Represents p < 0.05 (for detailed p value calculations, please see SI Table 6 ).
