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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [4]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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- Product number
- GTX100496 - Provider product page
- Provider
- GeneTex
- Proper citation
- GeneTex Cat#GTX100496, RRID:AB_1952592
- Product name
- XIAP antibody [C1C3]
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse
- Host
- Rabbit
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XIAP antibody [C1C3] detects XIAP protein by western blot analysis.A. 30 ?g 293T whole cell lysate/extract B. 30 ?g A431 whole cell lysate/extract C. 30 ?g H1299 whole cell lysate/extract7.5% SDS-PAGEXIAP antibody [C1C3] (GTX100496) dilution: 1:500 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Sample (30 ?g of whole cell lysate) A:NIH-3T37.5% SDS PAGE GTX100496 diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
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- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and XIAP knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XIAP antibody [C1C3] (GTX100496) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Wild-type (WT) and XIAP knockout (KO) HeLa cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with XIAP antibody [C1C3] (GTX100496) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XIAP antibody [C1C3] detects XIAP protein at cytoplasm by immunofluorescent analysis.Sample: HeLa cells were fixed in ice-cold MeOH for 5 min.Green: XIAP protein stained by XIAP antibody [C1C3] (GTX100496) diluted at 1:500.Blue: Hoechst 33342 staining.Scale bar = 10 £gm.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BIRC4(GTX100496) antibody at 1:500 dilution.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XIAP antibody [C1C3] detects XIAP protein at cytoplasm on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. XIAP antibody [C1C3] (GTX100496) diluted at 1:500.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- XIAP antibody [C1C3] detects XIAP protein at cytoplasm in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. XIAP antibody [C1C3] (GTX100496) diluted at 1:500.
