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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunoprecipitation [1]
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Validation data
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- Product number
- H00000331-D01 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#H00000331-D01, RRID:AB_10659918
- Product name
- XIAP MaxPab rabbit polyclonal antibody (D01)
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against a full-length human XIAP protein.
- Antigen sequence
MTFNSFEGSKTCVPADINKEEEFVEEFNRLKTFAN
FPSGSPVSASTLARAGFLYTGEGDTVRCFSCHAAV
DRWQYGDSAVGRHRKVSPNCRFINGFYLENSATQS
TNSGIQNGQYKVENYLGSRDHFALDRPSETHADYL
LRTGQVVDISDTIYPRNPAMYSEEARLKSFQNWPD
YAHLTPRELASAGLYYTGIGDQVQCFCCGGKLKNW
EPCDRAWSEHRRHFPNCFFVLGRNLNIRSESDAVS
SDRNFPNSTNLPRNPSMADYEARIFTFGTWIYSVN
KEQLARAGFYALGEGDKVKCFHCGGGLTDWKPSED
PWEQHAKWYPGCKYLLEQKGQEYINNIHLTHSLEE
CLVRTTEKTPSLTRRIDDTIFQNPMVQEAIRMGFS
FKDIKKIMEEKIQISGSNYKSLEVLVADLVNAQKD
SMQDESSQTSLQKEISTEEQLRRLQEEKLCKICMD
RNIAIVFVPCGHLVTCKQCAEAVDKCPMCYTVITF
KQKIFMS- Storage
- Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- BIRC4 MaxPab rabbit polyclonal antibody. Western Blot analysis of BIRC4 expression in HeLa.
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Western Blot analysis of XIAP expression in transfected 293T cell line (H00000331-T01) by XIAP MaxPab polyclonal antibody.Lane 1: XIAP transfected lysate(56.70 KDa).Lane 2: Non-transfected lysate.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunofluorescence of purified MaxPab antibody to XIAP on HeLa cell. [antibody concentration 10 ug/ml]
- Validation comment
- Immunofluorescence
- Protocol
- Protocol
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Immunoprecipitation of XIAP transfected lysate using anti-XIAP MaxPab rabbit polyclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with BIRC4 purified MaxPab mouse polyclonal antibody (B01P) (H00000331-B01P).
- Validation comment
- Immunoprecipitation
- Protocol
- Protocol
