Ab134877

antibody from Aladdin Scientific

Targeting: XIAP API3, BIRC4, hILP, ILP-1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

Ab134877

Provider

Aladdin Scientific

Product name

XIAP Antibody

Antibody type

Polyclonal

Description

Rabbit anti Human XIAP Antibody, Polyclonal, could be used for WB, IHC, IF/ICCand so on. Application: WB: 0.01-3µg/mL IHC: 2-30µg/mL IF/ICC: 2-30µg/mL Protein Function Apoptotic suppressor. Has E3 ubiquitin-protein ligase activity. Mediates the proteasomal degradation of target proteins, such as caspase-3, SMAC or AIFM1. Inhibitor of caspase-3, -7 and -9. Mediates activation of MAP3K7/TAK1, leading to the activation of NF-kappa-B.

Reactivity

Human, Mouse, Rat

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

AA 1-497


Vial size

100_l,10_l,1ml,20_l,50_l

Concentration

Lot by Lot

Storage

Store at 4_ short term (1-2 weeks). Store at -20_ long term (24 months). Upon delivery aliquot. Avoid freeze/thaw cycle.

Western blot

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Anti-XIAP antibody (Ab134877) - Western Blot All lanes: Anti-XIAP antibody (Ab134877) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Rabbit IgG H&L (HRP) (Ab170144) at 1/20000 dilution Predicted band size: 56 kDa Observed band size: 57 kDa Exposure time: 0.9 s

Immunocytochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Anti-XIAP antibody (Ab134877) - IF/ICC FITC staining on IF Samples: Human Hep G2 cells Primary Ab: 20 µg/mL Anti-XIAP antibody (Ab134877) Second Ab: 1 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Anti-XIAP antibody (Ab134877) - IF/ICC FITC staining on IF Samples: Human MCF7 cells Primary Ab: 20 μg/mL Anti-XIAP antibody (Ab134877) Second Ab: 1.5 μg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Anti-XIAP antibody (Ab134877) - IF/ICC FITC staining on IF Sample: MCF7 cells Primary Ab: 20 µg/mL Anti-XIAP antibody (Ab134877) Second Ab: 2 µg/mL FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Immunohistochemistry

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Anti-XIAP antibody (Ab134877) - IHC DAB staining on IHC Samples: Human Kidney Tissue Primary Ab: 30 µg/mL Anti-XIAP antibody (Ab134877) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Anti-XIAP antibody (Ab134877) - IHC DAB staining on IHC Samples: Human Breast cancer Tissue Primary Ab: 30 µg/mL Anti-XIAP antibody (Ab134877) Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Anti-XIAP antibody (Ab134877) - IHC DAB staining on IHC Sample: Human Breast cancer Tissue Primary Ab: 20 µg/mL Anti-XIAP antibody (Ab134877) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.

Supportive validation

Submitted by

Aladdin Scientific (provider)

Main image

Experimental details

Anti-XIAP antibody (Ab134877) - IHC DAB staining on IHC Sample: Human Kidney Tissue Primary Ab: 20 µg/mL Anti-XIAP antibody (Ab134877) Control: Used PBS instead of primary antibody Second Ab: 2 µg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody.