Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [2]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-27203 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- XIAP Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HeLa, 293T, A431, H1299, NIH-3T3. Predicted reactivity: Mouse (84%), Rat (82%), Pig (88%), Rabbit (85%), Bovine (83%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.78 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using XIAP Polyclonal Antibody (Product # PA5-27203). Sample (30 µg of whole cell lysate). Lane A: NIH-3T3. 7.5% SDS PAGE. XIAP Polyclonal Antibody (Product # PA5-27203) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- XIAP Polyclonal Antibody detects XIAP protein by western blot analysis. A. 30 µg 293T whole cell lysate/extract. B. 30 µg A431 whole cell lysate/extract. C. 30 µg H1299 whole cell lysate/extract.7.5% SDS-PAGE. XIAP Polyclonal Antibody (Product # PA5-27203) dilution: 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of BIRC4 showing staining in the cytoplasm of HeLa cells. HeLa cells were fixed in ice-cold MeOH for 5 min and stained using a BIRC4 polyclonal antibody (Product # PA5-27203) diluted at 1:500. Blue: Hoechst 33342 staining.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of XIAP was performed in HeLa cells fixed in ice-cold MeOH for 5 min. Green: XIAP Polyclonal Antibody (Product # PA5-27203) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry (Paraffin) analysis of XIAP was performed in paraffin-embedded human breast carcinoma tissue using XIAP Polyclonal Antibody (Product # PA5-27203) at a dilution of 1:500.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded SW480 xenograft, using BIRC4 (Product # PA5-27203) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- XIAP Polyclonal Antibody detects XIAP protein at cytoplasm on mouse spleen by immunohistochemical analysis. Sample: Paraffin-embedded mouse spleen. XIAP Polyclonal Antibody (Product # PA5-27203) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.