Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NB100-56184 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-56184, RRID:AB_839117
- Product name
- Rabbit Polyclonal XIAP Antibody
- Antibody type
- Polyclonal
- Description
- Unpurified. The antibody recognizes epitopes in the BIR2 domain of XIAP. Therefore it can recognize full-length XIAP and XIAP cleavage fragments containing the BIR2 domain. However, XIAP cleavage fragments may be biologically unstable, and therefore cleavage fragments may be difficult to detect.
- Reactivity
- Human, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.05 ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XIAP Antibody - Unpurified [NB100-56184] - Analysis of XIAP using this antibody to XIAP at 1:2000. Full-length recombinat XIAP was recognized; the antibody did not but not the other recombinant IAP proteins (e.g. cIAP1, cIAP2, survivin). The antibody detected full-length XIAP in human kidney tissue lysate.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: XIAP Antibody - Unpurified [NB100-56184] - Analysis of XIAP in various tumor cell lines using this antibody at 1:2000.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: XIAP Antibody - Unpurified [NB100-56184] - Analysis of XIAP in formalin-fixed, paraffin-embedded human esophageal carcinoma using this antibody at 1:2000. Hematoxylin-eosin counterstain.