MA1-10883

antibody from Invitrogen Antibodies

Targeting: TBP GTF2D1, SCA17, TFIID

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

MA1-10883

Provider

Invitrogen Antibodies

Product name

TBP Monoclonal Antibody (1TBP18)

Antibody type

Monoclonal

Antigen

Other

Description

MA1-10883 detects TATA-Binding Protein from human, mouse, and rat samples. It does not react with TBP from Drosophila, yeast, silkworms or xenopus.

Antibody clone number

1TBP18

Concentration

0.7 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of TBP was achieved by transfecting HeLa with TBP specific siRNAs (Silencer® select Product # s13826, s13827). Western blot analysis (Fig. a) was performed using nuclear enriched extracts from the TBP knockdown cells (lane 3), non-targeting scrambled siRNA transfected cells (lane 2) and untransfected cells (lane 1). The blot was probed with TBP Monoclonal Antibody (1TBP18) (Product # MA1-10883, 5 µg/mL concentration) and Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to TBP.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot was performed using Anti-TBP Monoclonal Antibody (1TBP18) (Product # MA1-10883) and a 41kDa band corresponding to TBP was observed across all cell lines except for HT-29 and mouse and rat testis tissues where a 37kDa band was observed along with other uncharacterized bands (*). Nuclear enriched extracts (30 µg lysate) of K-562 (Lane 1), HeLa (Lane 2), A-431 (Lane 3), HT-29 (Lane 4), NIH/3T3 (Lane 5), HEL 92.1.7 (Lane 6), Mouse Testis (Lane 7), Rat Testis (Lane 8) were electrophoresed using NuPAGE™ 10% Bis-Tris Protein Gel (Product # NP0302BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (5µg/mL concentration) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using SuperSignal™ West Dura Extended Duration Substrate (Product # 34076).