Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
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Validation data
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- Product number
- Ab130268 - Provider product page
- Provider
- Aladdin Scientific
- Product name
- TBP Mouse mAb
- Antibody type
- Monoclonal
- Description
- Mouse anti Human TBP Antibody, Monoclonal (C3), could be used for WB and so on.Application: WB: 0.5-2µg/mLProtein FunctionGeneral transcription factor that functions at the core of the DNA-binding multiprotein factor TFIID. Binding of TFIID to the TATA box is the initial transcriptional step of the pre-initiation complex (PIC), playing a role in the activation of eukaryotic genes transcribed by RNA polymerase II. Component of the transcription factor SL1/TIF-IB complex, which is involved in the assembly of the PIC (preinitiation complex) during RNA polymerase I-dependent transcription. The rate of PIC formation probably is primarily dependent on the rate of association of SL1 with the rDNA promoter. SL1 is involved in stabilization of nucleolar transcription factor 1/UBTF on rDNA.
- Reactivity
- Human, Mouse, Rat, Porcine
- Host
- Mouse
- Conjugate
- Unconjugated
- Antigen sequence
AA 25-339
- Antibody clone number
- C3
- Vial size
- 100_l,10_l,1ml,20_l,50_l
- Concentration
- Lot by Lot
- Storage
- Store at 4¡C short term (1-2 weeks). Store at -20¡C long term (12 months). Upon delivery aliquot. Avoid freeze/thaw cycle.
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Supportive validation
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- Aladdin Scientific (provider)
- Main image
- Experimental details
- TBP Mouse mAb (Ab130268) - Western Blot All lanes: TBP Mouse mAb (Ab130268) at 1/1000 dilution Samples: Lysates at 20 µg per lane Secondary: Goat Anti-Mouse IgG H&L (HRP) (Ab138040) at 1/10000 dilution Predicted band size: 38 kDa Observed band size: 43&36 kDa Exposure time: 5.1 s