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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [2]
- Immunohistochemistry [1]
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- Product number
- PA5-46959 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Uromodulin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse Uromodulin is observed.
- Concentration
- 0.2 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis from lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Product # PA5-46959) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody. A specific band was detected for Uromodulin at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Uromodulin in human kidney tissue. Samples were incubated in Uromodulin polyclonal antibody (Product # PA5-46959) using a dilution of 0.5 µg/mL followed by a HRP-conjugated Anti-Sheep IgG secondary antibody. A specific band was detected for Uromodulin at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Uromodulin in immersion fixed paraffin-embedded sections of human kidney. Samples were incubated in Uromodulin polyclonal antibody (Product # PA5-46959) using a dilution of 1.7 µg/mL overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules.
