20954-1-AP

antibody from Proteintech Group

Targeting: DNMT3A

Provider product page for 20954-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Antibody data

Antibody Data
Antigen structure
References [69]
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Validations
Western blot [1]
Immunocytochemistry [1]

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Product number: 20954-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#20954-1-AP, RRID:AB_10733339
Product name: DNMT3A antibody
Antibody type: Polyclonal
Description: DNMT3A antibody (Cat. #20954-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

DNMT3A protein structure - 20954-1-AP shown in red.

Submitted references Targeting CPS1 attenuates lung cancer metastasis by regulating EMT through an epigenetic mechanism.

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Chang A, Peng H, Chen Y, Zhang H, Xu X, Zhang K, Yang J, Li W, Wang X, Dong X, Ni J
Biomaterials 2026 Oct;333:124215

Molecular basis for the inhibition of de novo DNA methylation by TCL1A.

Liu Q, Li J, Wang X, Li Y, Wu Y, Han Z, Guo Z, Guo L, Wang X, Yuan G, Gao Z, Li L, Deng D
Nature communications 2026 Mar 5;17(1)

HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer.

Wang Y, Xiong Y, Liu S, Bao L, Qiu X, Korboukh I, Song X, Toffessi Tcheuyap V, Wu S, Chen M, Brugarolas J, Jin J, Wang Y, Luo W
Cell reports. Medicine 2026 Mar 17;7(3):102630

Cathepsin E drives colorectal cancer progression and immune evasion via ZNF326 ADP-ribosylation and TGF-β/SMAD activation.

Zhang H, Feng J, Zhu M, Shi T, Xi Q
Journal of translational medicine 2026 Mar 14;24(1)

Epigenetic reprogramming by organic arsenic CZ2 elicits potent antitumor responses in DLBCL through TMS1 restoration.

Mei W, Zhang M, Zhang D
Bioorganic chemistry 2026 Jun 15;174:109734

Discovery of a Novel DNMT1 Inhibitor with Improved Efficacy in Treating β-Thalassemia.

Shen Y, Wei J, Tang S, Wu D, Zong L, Ma S, Xiong Q, Gong R, Xu S, Peng C, Feng Q, Liu S, Liu Q, Ye Y, Zhao Q, Luo C, Huang P, Li Z, Kong X, Lan X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2026 Feb;13(11):e13469

UHRF1-mediated DNA 5-mC modification drives super-enhancer redistribution and impedes osteogenesis via TGM2-regulated autophagic flux in senile osteoporosis.

Pang P, Chen J, Li Q, Su Z, Lin J, Zeng Y, Zhang W, Xiao Z, Chen Z, Liu Z, Lin Y, Zheng G, Yu W, Xie Z
Journal of advanced research 2026 Apr 17;

DNMT3a promotes LUAD cell proliferation and metastasis by activating the HDAC7 signalling pathway.

Jiang M, Zhou X, Feng Y, Ding P, Li J, Lu D, Qin J, Bai Y, Wang A, Xia C, Wang J, Yan X, Ma Z, Zhang R
International journal of biological sciences 2025;21(4):1585-1602

Tianlong kechuanling ameliorated COPD-PH through DNMT3A-GSDMD axis-dependent regulation of endothelial cell pyroptosis.

Li H, Li Z, Gong J, Yan Y, Cui X, Yu Q, Shi Y, Tian X, Jiang Q, Tan Z, Yang Y, Zhou W, Ding Q, Wang P
Journal of ethnopharmacology 2025 Sep 25;353(Pt A):120332

Lactate-binding protein DNMT3A in HRMECs promotes angiogenesis by upregulating VEGFA through HIF-1α lactylation.

Wang X, Huang J, Fan W, Li N, Yang H, Yang W, Li W, Li R, Liu J, Li X, Zhou Q, Hou S
Genome biology 2025 Oct 30;26(1):377

PARP1 promoter hypermethylation promotes arsenic-induced skin damage by driving telomere dysfunction-mediated keratinocyte senescence.

Yang L, Wang W, Zhang A
Ecotoxicology and environmental safety 2025 Oct 1;304:119108

Arsenic promotes ROS-mediated malignant transformation of bronchial epithelial cells by specifically downregulating TXNL1 expression.

Zhao L, Jiao X, Li H, Chang Y, Li M, Liu Y, Xie Q, Huang C, Huang H
Communications biology 2025 Nov 22;8(1):1827

Klotho alleviates oxidative stress and mitochondrial dysfunction through the Nrf2/HO-1 pathway, thereby reducing renal senescence induced by calcium oxalate crystals.

Xu Y, You J, Yao J, Hou B, Wang W, Hao Z
Urolithiasis 2025 Mar 29;53(1):61

UHRF1 phase separation mediates stable inheritance of DNA methylation and promotes the proliferation of prostate cancer cells.

Li Z, Wang Y, Yu Z, Luo Y, Zhao Y, Xue E, Zhan J, Lu H, Sun F
Cell communication and signaling : CCS 2025 Jun 20;23(1):297

C1q(+) Macrophage-Tumor Cell Interaction Promoted Tumorigenesis via GPR17/PI3K/AKT Pathway Induced DNA Hypermethylation in Nasopharyngeal Carcinoma.

Liu Y, Huang C, Luo M, Lu W, Zhang B, Bai L, Zheng S, Tan Y, Li S, Wang H, Gong L, Guan X
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Jul;12(26):e2503434

IFNγ regulates ferroptosis in KFs by inhibiting the expression of SPOCD1 through DNMT3A.

Wang X, Yang Y, Zhou X, Yu S, Luo X, Lu L, Gao Z, Yang J
Cell death discovery 2025 Jan 16;11(1):9

Sequential Release HydroLipo System for STING Gene Epigenetic Reprogramming and Immune Activation in Glioblastoma.

Yu H, Liu W, Ding K, Wu J, Wang C, Wang S, Wu L, Tang Q, Yin X, Jiang K, Yan D, Wang X, Chen S, Yan S
Advanced science (Weinheim, Baden-Wurttemberg, Germany) 2025 Feb;12(5):e2408323

DNA methyltransferase 3A: A prognostic biomarker and potential target for immunotherapy in gastric cancer.

Wei Z, Kou Z, Luo Y, Cheng Y
Medicine 2025 Feb 14;104(7):e41578

Circular RNA Gsr-0002 promotes schistosomiasis-induced liver fibrosis by regulating the DNMT3A/PTEN pathway.

Dai B, Huang S, Hu Q, Wang P, Gong L, Xu G, Wu F, Qin H, Xia S, Sun W, Li H, Feng Z, Ren C, Shen J, Liu M
International journal of biological macromolecules 2025 Dec;333(Pt 1):148776

DNMT3a Deficiency Contributes to Anesthesia/Surgery-Induced Synaptic Dysfunction and Cognitive Impairment in Aged Mice.

Cong P, Huang X, Zhang Q, He M, Wan H, Wu Q, Wu H, Zhang Y, Cheng C, Tian L, Xiong L
Aging cell 2025 Apr;24(4):e14458

Impaired TFEB-mediated autophagy-lysosome fusion promotes tubular cell cycle G2/M arrest and renal fibrosis by suppressing ATP6V0C expression and interacting with SNAREs.

Ren X, Wang J, Wei H, Li X, Tian Y, Wang Z, Yin Y, Guo Z, Qin Z, Wu M, Zeng X
International journal of biological sciences 2024;20(5):1905-1926

GRP78/IRE1 and cGAS/STING pathway crosstalk through CHOP facilitates iodoacetic acid-mediated testosterone decline.

Mou L, Sun D, Qu J, Tan X, Wang S, Zeng Q, Liu C
Journal of hazardous materials 2024 Sep 5;476:135101

The up-regulation of SYNCRIP promotes the proliferation and tumorigenesis via DNMT3A/p16 in colorectal cancer.

Li C, Lu T, Chen H, Yu Z, Chen C
Scientific reports 2024 Sep 16;14(1):21570

An engineered cellular carrier delivers miR-138-5p to enhance mitophagy and protect hypoxic-injured neurons via the DNMT3A/Rhebl1 axis.

Zhu X, Liu Q, Zhu F, Jiang R, Lu Z, Wang C, Gong P, Yao Q, Xia T, Sun J, Ju F, Wang D, Sun R, Zhou Y, You B, Shi W
Acta biomaterialia 2024 Sep 15;186:424-438

Prenatal exposure to low-dose bisphenol A disrupts hippocampal DNA methylation and demethylation in male rat offspring.

Wang Y, Guo Y, Ren J, Liu Q, Wang C
Toxicology and industrial health 2024 Jul;40(7):376-386

Methylation of eNOS in the rat penile corpus cavernosum under different pathological states and its relationship with erectile function.

Wang N, Jiang Q, Xie L, Cheng B, Liu QW, Jiang R
Andrology 2024 Jan;12(1):222-230

miR-29c-3p acts as a tumor promoter by regulating β-catenin signaling through suppressing DNMT3A, TET1 and HBP1 in ovarian carcinoma.

Zhao H, Feng L, Cheng R, Wu M, Bai X, Fan L, Liu Y
Cellular signalling 2024 Jan;113:110936

Blocking the interaction between circTNRC18 and LIN28A promotes trophoblast epithelial-mesenchymal transformation and alleviates preeclampsia.

Chen LL, Li YQ, Kang ZH, Zhang X, Gu SY, Wang N, Shen XY
Molecular and cellular endocrinology 2024 Jan 1;579:112073

HOXD1 inhibits lung adenocarcinoma progression and is regulated by DNA methylation.

Hu X, Zhang S, Zhang X, Liu H, Diao Y, Li L
Oncology reports 2024 Dec;52(6)

DNMT3A loss drives a HIF-1-dependent synthetic lethality to HDAC6 inhibition in non-small cell lung cancer.

Zhang J, Zhao Y, Liang R, Zhou X, Wang Z, Yang C, Gao L, Zheng Y, Shao H, Su Y, Cui W, Jia L, Yang J, Wu C, Wang L
Acta pharmaceutica Sinica. B 2024 Dec;14(12):5219-5234

Promotor Hypomethylation Mediated Upregulation of VCAN Targets Twist1 to Promote EndMT in Hypoxia-Induced Pulmonary Hypertension.

Yu J, Hong S, Yang L, Ye S, Yu Z, Zhang Z, Wang Z, Huang S, Chen Y, Bian T, Wu Y
Journal of the American Heart Association 2024 Dec 3;13(23):e036969

TET3-facilitated differentiation of human umbilical cord mesenchymal stem cells into oligodendrocyte precursor cells for spinal cord injury recovery.

Zhang Y, Peng Z, Guo M, Wang Y, Liu J, Liu Y, Li M, Wei T, Li P, Zhao Y, Wang Y
Journal of translational medicine 2024 Dec 20;22(1):1118

Maternal high-fat diet orchestrates offspring hepatic cholesterol metabolism via MEF2A hypermethylation-mediated CYP7A1 suppression.

Zhang L, Zou W, Zhang S, Wu H, Gao Y, Zhang J, Zheng J
Cellular & molecular biology letters 2024 Dec 18;29(1):154

Epigenetic repression of de novo cysteine synthetases induces intra-cellular accumulation of cysteine in hepatocarcinoma by up-regulating the cystine uptake transporter xCT.

Yamauchi T, Okano Y, Terada D, Yasukochi S, Tsuruta A, Tsurudome Y, Ushijima K, Matsunaga N, Koyanagi S, Ohdo S
Cancer & metabolism 2024 Aug 7;12(1):23

Inhibiting DNA methyltransferase DNMT3B confers protection against ferroptosis in nucleus pulposus and ameliorates intervertebral disc degeneration via upregulating SLC40A1.

Chen J, Yang X, Li Q, Ma J, Li H, Wang L, Chen Z, Quan Z
Free radical biology & medicine 2024 Aug 1;220:139-153

DNMT3A promotes glioma growth and malignancy via TNF-α/NF-κB signaling pathway.

Su X, Liu J, Tu Z, Ji Q, Li J, Liu F
Translational cancer research 2024 Apr 30;13(4):1786-1806

Vpr driving DNA methylation variation of CD4 + T cells in HIV-1 infection.

Wang P, Meng Z, Deng K, Gao Z, Cai J
Virology journal 2024 Apr 26;21(1):97

miR-29b-3p regulates cardiomyocytes pyroptosis in CVB3-induced myocarditis through targeting DNMT3A.

Wang Y, Zhang Z, Li H, Wang M, Qiu Y, Lu L
Cellular & molecular biology letters 2024 Apr 20;29(1):55

DNMT3A-mediated epigenetic silencing of SOX17 contributes to endothelial cell migration and fibroblast activation in wound healing.

Yu X, Ma X, Zhou J
PloS one 2023;18(10):e0292684

Differential expression of epigenetic modifiers in early and late cardiotoxic heart failure reveals DNA methylation as a key regulator of cardiotoxicity.

Robinson EL, Ameri P, Delrue L, Vanderheyden M, Bartunek J, Altieri P, Heymans S, Heggermont WA
Frontiers in cardiovascular medicine 2023;10:884174

LINC00955 suppresses colorectal cancer growth by acting as a molecular scaffold of TRIM25 and Sp1 to Inhibit DNMT3B-mediated methylation of the PHIP promoter.

Ren G, Li H, Hong D, Hu F, Jin R, Wu S, Sun W, Jin H, Zhao L, Zhang X, Liu D, Huang C, Huang H
BMC cancer 2023 Sep 23;23(1):898

Hypermethylation of microRNA-497-3p contributes to progression of thyroid cancer through activation of PAK1/β-catenin.

Fan Y, Fan X, Yan H, Liu Z, Wang X, Yuan Q, Xie J, Lu X, Yang Y
Cell biology and toxicology 2023 Oct;39(5):1979-1994

The Notch1/Hes1 signaling pathway affects autophagy by adjusting DNA methyltransferases expression in a valproic acid-induced autism spectrum disorder model.

Li Y, Ma L, Deng Y, Du Z, Guo B, Yue J, Liu X, Zhang Y
Neuropharmacology 2023 Nov 15;239:109682

Base editing-mediated one-step inactivation of the Dnmt gene family reveals critical roles of DNA methylation during mouse gastrulation.

Li Q, Lu J, Yin X, Chang Y, Wang C, Yan M, Feng L, Cheng Y, Gao Y, Xu B, Zhang Y, Wang Y, Cui G, Xu L, Sun Y, Zeng R, Li Y, Jing N, Xu GL, Wu L, Tang F, Li J
Nature communications 2023 May 22;14(1):2922

Effects of H19/SAHH/DNMT1 on the oxidative DNA damage related to benzo[a]pyrene exposure.

Fu Y, Li X, Pan B, Niu Y, Zhang B, Zhao X, Nie J, Yang J
Environmental science and pollution research international 2023 Jan;30(5):11706-11718

Epigenetic control of LncRNA NEAT1 enables cardiac fibroblast pyroptosis and cardiac fibrosis.

Ding JF, Zhou Y, Xu SS, Shi KH, Sun H, Tu B, Song K, Xuan HY, Sha JM, Zhao JY, Tao H
European journal of pharmacology 2023 Jan 5;938:175398

Decreased expression and DNA hypermethylation of syncytin-1 in human villus tissues with unexplained recurrent spontaneous abortion.

Fu Y, Song Y, Zhang J, Wei LP, Sun XR
Journal of reproductive immunology 2023 Feb;155:103784

TDG suppresses the migration and invasion of human colon cancer cells via the DNMT3A/TIMP2 axis.

Miao J, Zhao C, Tang K, Xiong X, Wu F, Xue W, Duan B, Zhang H, Jing X, Li W, Sun Y, Hou N, Huang C
International journal of biological sciences 2022;18(6):2527-2539

SIRT1 modifies DNA methylation linked to synaptic deficits induced by Pb in vitro and in vivo.

Wang R, Yang M, Wu Y, Liu R, Liu M, Li Q, Su X, Xin Y, Huo W, Deng Q, Ba Y, Huang H
International journal of biological macromolecules 2022 Sep 30;217:219-228

m6A Methyltransferase METTL3 Reduces Hippocampal Neuron Apoptosis in a Mouse Model of Autism Through the MALAT1/SFRP2/Wnt/β-catenin Axis.

Ming Y, Deng Z, Tian X, Jia Y, Ning M, Cheng S
Psychiatry investigation 2022 Oct;19(10):771-787

CircDOCK1 Regulates miR-186/DNMT3A to Promote Osteosarcoma Progression.

Jin Z, Ye J, Chen S, Ren Y, Guo W
Biomedicines 2022 Nov 23;10(12)

Tumor suppressor CEBPA interacts with and inhibits DNMT3A activity.

Chen X, Zhou W, Song RH, Liu S, Wang S, Chen Y, Gao C, He C, Xiao J, Zhang L, Wang T, Liu P, Duan K, Cheng Z, Zhang C, Zhang J, Sun Y, Jackson F, Lan F, Liu Y, Xu Y, Wong JJ, Wang P, Yang H, Xiong Y, Chen T, Li Y, Ye D
Science advances 2022 Jan 28;8(4):eabl5220

Epigenetic integrity of paternal imprints enhances the developmental potential of androgenetic haploid embryonic stem cells.

Zhang H, Li Y, Ma Y, Lai C, Yu Q, Shi G, Li J
Protein & cell 2022 Feb;13(2):102-119

CircNTNG1 inhibits renal cell carcinoma progression via HOXA5-mediated epigenetic silencing of Slug.

Liang Y, Cen J, Huang Y, Fang Y, Wang Y, Shu G, Pan Y, Huang K, Dong J, Zhou M, Xu Y, Luo J, Liu M, Zhang J
Molecular cancer 2022 Dec 19;21(1):224

piRNA-823 Is Involved in Cancer Stem Cell Regulation Through Altering DNA Methylation in Association With Luminal Breast Cancer.

Ding X, Li Y, Lü J, Zhao Q, Guo Y, Lu Z, Ma W, Liu P, Pestell RG, Liang C, Yu Z
Frontiers in cell and developmental biology 2021;9:641052

Exhausting circ_0136474 and Restoring miR-766-3p Attenuate Chondrocyte Oxidative Injury in IL-1β-Induced Osteoarthritis Progression Through Regulating DNMT3A.

Zhu H, Zhu S, Shang X, Meng X, Jing S, Yu L, Deng Y
Frontiers in genetics 2021;12:648709

Downregulation of DNMT3a expression by RNAi and its effect on NF-κBs expression of thymic epithelial cells.

Meng FJ, Guo F, Sun ZN, Wang SJ, Yang CR, Wang CY, Zhang WC, Gao ZY, Ji LL, Feng FK, Guan ZY, Wang GS
Immunology letters 2021 Sep;237:17-26

Methylation gene KCNC1 is associated with overall survival in patients with seminoma.

Chen S, Xiao L, Peng H, Wang Z, Xie J
Oncology reports 2021 May;45(5)

Coeloglossum viride var. bracteatum extract attenuates staurosporine induced neurotoxicity by restoring the FGF2-PI3K/Akt signaling axis and Dnmt3.

Cai ZP, Cao C, Guo Z, Yu Y, Zhong SJ, Pan RY, Liang H, Lan R, Qin XY
Heliyon 2021 Jul;7(7):e07503

Huaier Inhibits Proliferation, Migration, and Invasion of Cutaneous Squamous Cell Carcinoma Cells by Inhibiting the Methylation Levels of CDKN2A and TP53.

Wang L, Xu L, Wang Y
Integrative cancer therapies 2021 Jan-Dec;20:15347354211031646

DNMT1-mediated PPARα methylation aggravates damage of retinal tissues in diabetic retinopathy mice.

Zhu Y, Wang X, Zhou X, Ding L, Liu D, Xu H
Biological research 2021 Aug 6;54(1):25

Neuroprotective Effect of Piracetam against Cocaine-Induced Neuro Epigenetic Modification of DNA Methylation in Astrocytes.

Sivalingam K, Samikkannu T
Brain sciences 2020 Sep 5;10(9)

Upregulation of programmed death ligand 1 by liver kinase B1 and its implication in programmed death 1 blockade therapy in non-small cell lung cancer.

Shen X, Zhao Y, Liu G, Zhou HL, Fan J, Zhang L, Li YL, Wang Y, Liang J, Xu ZX
Life sciences 2020 Sep 1;256:117923

Developmental nicotine exposure engenders intergenerational downregulation and aberrant posttranslational modification of cardinal epigenetic factors in the frontal cortices, striata, and hippocampi of adolescent mice.

Buck JM, O'Neill HC, Stitzel JA
Epigenetics & chromatin 2020 Mar 5;13(1):13

Radiation-Induced DNMT3B Promotes Radioresistance in Nasopharyngeal Carcinoma through Methylation of p53 and p21.

Wu C, Guo E, Ming J, Sun W, Nie X, Sun L, Peng S, Luo M, Liu D, Zhang L, Mei Q, Long G, Hu G, Hu G
Molecular therapy oncolytics 2020 Jun 26;17:306-319

LncRNA NKILA regulates endothelium inflammation by controlling a NF-κB/KLF4 positive feedback loop.

Zhu X, Du J, Yu J, Guo R, Feng Y, Qiao L, Xu Z, Yang F, Zhong G, Liu F, Cheng F, Chu M, Lin J
Journal of molecular and cellular cardiology 2019 Jan;126:60-69

Epigenetic modification differences between fetal fibroblast cells and mesenchymal stem cells of the Arbas Cashmere goat.

Wang X, Wang Z, Wang Q, Wang H, Liang H, Liu D
Research in veterinary science 2017 Oct;114:363-369

miR-194 functions as a novel modulator of cellular senescence in mouse embryonic fibroblasts.

Xu S, Zhang B, Zhu Y, Huang H, Yang W, Huang H, Zheng HL, Liu X
Cell biology international 2017 Mar;41(3):249-257

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HEK-293 cells were subjected to SDS PAGE followed by western blot with 20954-1-AP(DNMT3A antibody) at dilution of 1:1500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of HepG2 cells, using DNMT3A antibody 20954-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line