GTX30365

antibody from GeneTex

Targeting: DNMT3A

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

GTX30365

Provider

GeneTex

Proper citation

GeneTex Cat#GTX30365, RRID:AB_385509

Product name

DNMT3A antibody

Antibody type

Polyclonal

Reactivity

Human, Mouse

Host

Rabbit

References

Submitted references

Phospho-ΔNp63α/microRNA network modulates epigenetic regulatory enzymes in squamous cell carcinomas.

Ratovitski EA
Cell cycle (Georgetown, Tex.) 2014;13(5):749-61

---

DNMT1 is required to maintain CpG methylation and aberrant gene silencing in human cancer cells.

Robert MF, Morin S, Beaulieu N, Gauthier F, Chute IC, Barsalou A, MacLeod AR
Nature genetics 2003 Jan;33(1):61-5

---

An essential role for DNA methyltransferase DNMT3B in cancer cell survival.

Beaulieu N, Morin S, Chute IC, Robert MF, Nguyen H, MacLeod AR
The Journal of biological chemistry 2002 Aug 2;277(31):28176-81

Western blot

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Detection of overexpressed DNMT3A level in HCT-116 cells. Lane 1: 40 μg of nuclear extract from untreated HCT-116 cells. Lane 2: 40 μg of Nuclear extract from HCT-116 cells overexpressing DNMT3A.

Immunocytochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: Dnmt3a Antibody [GTX30365] - Dnmt3a antibody was tested in Ntera-2 cells with Dylight 488 (green). Nuclei and beta-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

Immunohistochemistry

Supportive validation

Submitted by

GeneTex (provider)

Main image

Experimental details

Immunohistochemistry: Dnmt3a Antibody [GTX30365] - Dnmt3a antibody was tested in human testis using DAB with hematoxylin counterstain.