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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [5]
- Immunoprecipitation [2]
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Validation data
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- Product number
- GTX129126 - Provider product page
- Provider
- GeneTex
- Product name
- DNMT3A antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
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Enhanced validation
Supportive validation
- Submitted by
- GeneTex (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129126) diluted at 1:3000.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3A antibody detects DNMT3A protein by western blot analysis.A. 30 £gg 293T whole cell extract B. 30 £gg 293T nuclear extract5 % SDS-PAGEDNMT3A antibody (GTX129126) dilution: 1:5000
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Non-transfected (¡V) and transfected (+) 293T whole cell extracts (30 ?g) were separated by 5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129126) diluted at 1:3000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Whole cell extract (30 ?g) was separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with DNMT3A antibody (GTX129126) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- DNMT3A antibody immunoprecipitates DNMT3A protein in IP experiments.IP samples: 293T nuclear extractA. 40 £gg 293T nuclear extractB. Control with 4 £gg of preimmune Rabbit IgGC. Immunoprecipitation of DNMT3A protein by 4 £gg DNMT3A antibody (GTX129126)5 % SDS-PAGEThe immunoprecipitated DNMT3A protein was detected by DNMT3A antibody (GTX129126) diluted at 1:2000.[EasyBlot anti-rabbit IgG (GTX221666-01) was used as a secondary reagent]
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Immunoprecipitation of DNMT3A protein from 293T whole cell extracts using 5 £gg of DNMT3A antibody (GTX129126) or DNMT3A antibody (GTX129125).Western blot analysis was performed using DNMT3A antibody (GTX129126).EasyBlot anti-Rabbit IgG (GTX221666-01) was used as a secondary reagent.
