Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [1]
- Immunocytochemistry [1]
- Immunohistochemistry [3]
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Validation data
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- Product number
- PA5-91951 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- DNMT3A Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- Positive Samples: Mouse spleen, Mouse skeletal muscle; Cellular Location: Cytoplasm, Nucleus
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 3.77 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of DNMT3A in extracts of various cell lines using DNMT3A Polyclonal Antibody (Product # PA5-91951) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry-Immunofluorescence analysis of DNMT3A was performed in A549 cells using DNMT3A Polyclonal Antibody (Product # PA5-91951).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DNMT3A in paraffin-embedded rat brain using DNMT3A Polyclonal Antibody (Product # PA5-91951) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DNMT3A in paraffin-embedded human kidney using DNMT3A Polyclonal Antibody (Product # PA5-91951) at a dilution of 1:100.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemistry analysis of DNMT3A in paraffin-embedded mouse heart using DNMT3A Polyclonal Antibody (Product # PA5-91951) at a dilution of 1:200.