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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- ELISA [1]
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- Product number
- PAB14074 - Provider product page
- Provider
- Abnova Corporation
- Proper citation
- Abnova Corporation Cat#PAB14074, RRID:AB_10552120
- Product name
- Dnmt3a polyclonal antibody
- Antibody type
- Polyclonal
- Description
- Rabbit polyclonal antibody raised against synthetic peptide of Dnmt3a.
- Storage
- Store at -20°C.Aliquot to avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- Nuclear extracts of HeLa cells (40 ug) were analysed by Western blot using Dnmt3a polyclonal antibody (Cat # PAB14074) diluted 1 : 500 in TBS-Tween containing 5% skimmed milk.
Supportive validation
- Submitted by
- Abnova Corporation (provider)
- Main image
- Experimental details
- ELISA was performed using a serial dilution of Dnmt3a polyclonal antibody (Cat # PAB14074).The wells were coated with the peptides used for immunisation of the rabbit.By plotting the absorbance against the antibody dilution, the titer of the purified antibody was estimated to be 1 : 86,500.
- Validation comment
- ELISA
