MA5-16171

antibody from Invitrogen Antibodies

Targeting: DNMT3A

Western blot

Immunohistochemistry

Chromatin Immunoprecipitation

Antibody data

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Validation data

Product number: MA5-16171 - Provider product page
Provider: Invitrogen Antibodies
Product name: DNMT3A Monoclonal Antibody (64B1446)
Antibody type: Monoclonal
Antigen: Recombinant full-length protein
Description: In Western blot, this antibody detects a band at approximately 120 kDa (predicted molecular weight: 102 kDa). Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min. Suggested positive control: antigen standard for DNMT3A (transient overexpression lysate), transfected cell lysate (WB), HeLa cells (ICC).
Reactivity: Human, Mouse, Rat
Host: Mouse
Isotype: IgG
Antibody clone number: 64B1446
Vial size: 100 µg
Concentration: 1 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

DNMT3A protein structure - MA5-16171 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of DNMT3A in NTERA-2 cells (blue) and a matched isotype control (orange). Samples were incubated in DNMT3A monoclonal antibody (Product # MA5-16171) using a dilution of 1 µg/mL for 30 minutes at room temperature. Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Phycoerythrin.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of DNMT3A in HepG2 cells. Samples were incubated in DNMT3A monoclonal antibody (Product # MA5-16171) using a dilution of 1 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to allophycocyanin (APC).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of DNMT3A in HeLa cells. Samples were incubated in DNMT3A monoclonal antibody (Product # MA5-16171) using a dilution of 1 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Both antibodies were conjugated to allophycocyanin (APC).

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of DNMT3A in Jurkat cells. Samples were incubated in DNMT3A monoclonal (Product # MA5-16171) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. Antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeablized with 0.1% saponin. Both antibodies were conjugated to Alexa Fluor 647.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Flow cytometry of DNMT3A in 1 x 10^6 CHO (A) and HEK-293 (B) cells. Samples were incubated in DNMT3A monoclonal antibody (Product # MA5-16171) using a dilution of 1 µg/1x10^6 cells. Antibody (dark blue). Isotype control shown in orange.