PA5-34692

antibody from Invitrogen Antibodies

Targeting: CAST

Western blot (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-34692

Provider

Invitrogen Antibodies

Product name

Calpastatin Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Recombinant protein fragment

Description

Recommended positive controls: A549.

Concentration

0.7 mg/mL

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western Blot using Calpastatin Polyclonal Antibody (Product # PA5-34692). Sample (30 µg of whole cell lysate). Lane A: A549 . 7.5% SDS PAGE. Calpastatin Polyclonal Antibody (Product # PA5-34692) diluted at 1:2,000.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Knockdown of Calpastatin was achieved by transfecting HeLa cells with Calpastatin specific siRNAs (Silencer® select Product # s2398, s2399). Western blot analysis (Fig. a) was performed using whole cell extracts from Calpastatin knockdown cells (Lane 3), non-specific scrambled siRNA transfected cells (Lane 2) and untransfected cells (Lane 1). The blot was probed with Calpastatin Polyclonal Antibody (Product # PA5-34692, 1:500 dilution) and Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution). Densitometric analysis of this western blot is shown in histogram (Fig. b). Decrease in signal upon siRNA mediated knock down confirms that antibody is specific to Calpastatin.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of Calpastatin was performed in paraffin-embedded human lung cancer tissue using Calpastatin Polyclonal Antibody (Product # PA5-34692) at a dilution of 1:500.