Antibody data
- Antibody Data
 - Antigen structure
 - References [0]
 - Comments [0]
 - Validations
 - Western blot [1]
 - ELISA [1]
 - Immunocytochemistry [1]
 
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Validation data
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- Product number
 - H00000831-M03 - Provider product page

 - Provider
 - Novus Biologicals
 - Proper citation
 - Novus Cat#H00000831-M03, RRID:AB_1260595
 - Product name
 - Mouse Monoclonal Calpastatin Antibody
 - Antibody type
 - Monoclonal
 - Description
 - IgG purified. CAST - calpastatin (2C5)
 - Reactivity
 - Human, Mouse
 - Host
 - Mouse
 - Isotype
 - IgG
 - Vial size
 - 0.1 mg
 - Storage
 - Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
 
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					Supportive validation
					
									
				
				- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Western Blot: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of CAST expression in NIH/3T3 (Cat # L018V1).
 
							
					Supportive validation
					
									
				
				- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Sandwich ELISA: Calpastatin Antibody (2C5) [H00000831-M03] - Detection limit for recombinant GST tagged CAST is 0.03 ng/ml as a capture antibody.
 
							
					Supportive validation
					
									
				
		- Submitted by
 - Novus Biologicals (provider)
 - Main image
 
- Experimental details
 - Immunocytochemistry/Immunofluorescence: Calpastatin Antibody (2C5) [H00000831-M03] - Analysis of monoclonal antibody to CAST on HeLa cell. Antibody concentration 10 ug/ml