MA3-945

antibody from Invitrogen Antibodies

Targeting: CAST

Western blot

Antibody data

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Validation data

Product number: MA3-945 - Provider product page
Provider: Invitrogen Antibodies
Product name: Calpastatin Monoclonal Antibody (2G11D6)
Antibody type: Monoclonal
Antigen: Other
Description: MA3-945 detects calpastatin from bovine, human, canine, pig, rabbit, and rat tissues and cells. This antibody does not cross-react with calpains or calmodulin. MA3-945 has been successfully used in Western blot, immunohistochemistry, and immunocytochemical procedures. By Western blot, this antibody detects 150, 125, 90 and 70 kDa proteins which have been suggested to be proteins translated from different start sites of the calpastatin gene. Immunofluorescence staining of calpastatin in porcine LLC-PK1 cells with MA3-945 results in diffuse cytoplasmic staining and weak nuclear staining. The MA3-945 antigen is bovine skeletal muscle calpastatin. This antibody recognizes an epitope found between amino acid residues 295-501 (domain II) of human calpastatin.
Reactivity: Human, Rat, Bovine, Canine, Porcine, Rabbit
Host: Mouse
Isotype: IgG
Antibody clone number: 2G11D6
Vial size: 100 µL
Concentration: Conc. Not Determined
Storage: -20°C, Avoid Freeze/Thaw Cycles

CAST protein structure - MA3-945 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on cancer biopsies of deparaffinized Human breast carcinoma tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:1000 with a mouse monoclonal antibody recognizing Calpastatin (Product # MA3-945) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on biopsies of deparaffinized Human skeletal muscle tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:200 with a mouse monoclonal antibody recognizing Calpastatin (Product # MA3-945) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: Immunohistochemistry was performed on normal biopsies of deparaffinized Human tonsil tissue. To expose target proteins, heat induced antigen retrieval was performed using 10mM sodium citrate (pH6.0) buffer, microwaved for 8-15 minutes. Following antigen retrieval tissues were blocked in 3% BSA-PBS for 30 minutes at room temperature. Tissues were then probed at a dilution of 1:500 with a mouse monoclonal antibody recognizing Calpastatin (Product # MA3-945) or without primary antibody (negative control) overnight at 4°C in a humidified chamber. Tissues were washed extensively with PBST and endogenous peroxidase activity was quenched with a peroxidase suppressor. Detection was performed using a biotin-conjugated secondary antibody and SA-HRP, followed by colorimetric detection using DAB. Tissues were counterstained with hematoxylin and prepped for mounting.