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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunocytochemistry [1]
- Flow cytometry [1]
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- Product number
- MA5-34787 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- TYROBP Recombinant Rabbit Monoclonal Antibody (JG38-70)
- Antibody type
- Monoclonal
- Antigen
- Recombinant full-length protein
- Description
- Positive Control: THP-1, K562, human lung cancer tissue.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Antibody clone number
- JG38-70
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- -20° C, Avoid Freeze/Thaw Cycles, store in dark
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence analysis of TYROBP is performed using THP-1 cells and THP1 differentiated to Macrophages. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with TYROBP Recombinant Rabbit Monoclonal Antibody (JG38-70) (Product # MA5-34787) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A32790, 1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b: Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained withAlexa Fluor™ 488 Phalloidin (Product # A12379, 1:300). Panel d represents the merged image showing Plasma Membrane localization. Panel e represents THP1 undifferentiated cells. Panel f represents control cells with no primary antibody to assess background. The images were captured at 60X magnification.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Flow cytometry of DAP12 in K562 cells (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Samples were incubated with DAP12 monoclonal antibody (Product # MA5-34787) at a dilution of 1:100, followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG.
