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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [3]
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- Product number
- GTX132808 - Provider product page
- Provider
- GeneTex
- Product name
- IRE1 alpha (phospho Ser724) antibody
- Antibody type
- Polyclonal
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
Submitted references Naphthoquinone derivative PPE8 induces endoplasmic reticulum stress in p53 null H1299 cells.
Lien JC, Huang CC, Lu TJ, Tseng CH, Sung PJ, Lee HZ, Bao BY, Kuo YH, Lu TL
Oxidative medicine and cellular longevity 2015;2015:453679
Oxidative medicine and cellular longevity 2015;2015:453679
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Supportive validation
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Untreated (¡V) and treated (+) HepG2 whole cell extracts (20 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 (phospho Ser724) antibody (GTX132808) diluted at 1:1000.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
- Submitted by
- GeneTex (provider)
- Main image
- Experimental details
- Various whole cell extracts (30 ?g) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha (phospho Ser724) antibody (GTX132808) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody (GTX213110-01) was used to detect the primary antibody.
