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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [2]
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- Validations
- Western blot [5]
- Immunocytochemistry [3]
- Immunohistochemistry [4]
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- Product number
- PA5-20189 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRE1 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is A-20 cell lysate.
- Concentration
- 1 mg/mL
Submitted references Featured Article: Deterioration of visual function mediated by senescence-associated endoplasmic reticulum stress in inflammatory tie2-TNF mice.
Acetyl-l-carnitine prevents homocysteine-induced suppression of Nrf2/Keap1 mediated antioxidation in human lens epithelial cells.
Lenin R, Nagy PG, Gentry J, Gangaraju R
Experimental biology and medicine (Maywood, N.J.) 2018 Aug;243(12):976-984
Experimental biology and medicine (Maywood, N.J.) 2018 Aug;243(12):976-984
Acetyl-l-carnitine prevents homocysteine-induced suppression of Nrf2/Keap1 mediated antioxidation in human lens epithelial cells.
Yang SP, Yang XZ, Cao GP
Molecular medicine reports 2015 Jul;12(1):1145-50
Molecular medicine reports 2015 Jul;12(1):1145-50
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of A-20 cell lysate using a IRE1p polyclonal antibody (Product # PA5-20189) at (A) 0.5, (B) 1 and (C) 2 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of IRE1-alpha in HeLa WT (Lane 1) or IRE1P KO (Lane 2) cells. Lysates (10 µg) were loaded onto SDS-PAGE and blots were probed with IRE1 alpha Polyclonal Antibody (Product # PA5-20189) diluted to 0.5 µg/mL and anti-beta actin diluted to 1 µg/mL. 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Human Cell Lines. Loading: 15 µg of lysates per lane. Antibodies: IRE1 alpha Polyclonal Antibody (Product # PA5-20189) (0.4 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution. Lane 1: Caco-2, Lane2: SK-N-SH
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Mouse A20 Cell Lysate. Loading: 15 µg of lysates per lane. Antibodies: IRE1 alpha Polyclonal Antibody (Product # PA5-20189) (A: 0.5 µg/mL, B: 1 µg/mL, C: 2 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot Validation in Rat Brain Tissue Lysate. Loading: 15 µg of lysates per lane. Antibodies: IRE1 alpha Polyclonal Antibody (Product # PA5-20189) (A: 0.5 µg/mL, B: 1 µg/mL), 1h incubation at RT in 0.05 NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A20 cells using a IRE1p polyclonal antibody (Product # PA5-20189) at a 2 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of A20 cells using IRE1 alpha Polyclonal Antibody (Product # PA5-20189) at 1 µg/mL. Cells were fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1:250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed A20 Cells labeling IRE1P with IRE1 alpha Polyclonal Antibody (Product # PA5-20189) at 2 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (red).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Human Small Intestine Tissue labeling IRE1p with IRE1 alpha Polyclonal Antibody (Product # PA5-20189) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of 4% paraformaldehyde-fixed Rat Small Intestine Tissue labeling IRE1p with IRE1 alpha Polyclonal Antibody (Product # PA5-20189) at 20 µg/mL, followed by goat anti-rabbit IgG secondary antibody at 1:500 dilution (green) and DAPI staining (blue).
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Human Small Intestine Tissue using IRE1 alpha Polyclonal Antibody (Product # PA5-20189) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded Rat Small Intestine Tissue using IRE1 alpha Polyclonal Antibody (Product # PA5-20189) at 2 µg/mL. Tissue was fixed with formaldehyde and blocked with 0.1 serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
