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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Immunocytochemistry [5]
- Other assay [1]
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- Product number
- PA5-20190 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRE1 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- A suggested positive control is A-20 cell lysate. PA5-20190 can be used with blocking peptide PEP-0320.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 1 mg/mL
- Storage
- Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C
Submitted references Effect of Propionic Acid on Diabetes-Induced Impairment of Unfolded Protein Response Signaling and Astrocyte/Microglia Crosstalk in Rat Ventromedial Nucleus of the Hypothalamus.
Natrus LV, Osadchuk YS, Lisakovska OO, Labudzinskyi DO, Klys YG, Chaikovsky YB
Neural plasticity 2022;2022:6404964
Neural plasticity 2022;2022:6404964
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescent analysis of A20 cells using a IRE1p polyclonal antibody (Product # PA5-20190) at a 10 µg/mL dilution.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of IRE1p in A20 cells with IRE1 alpha Polyclonal Antibody (Product # PA5-20190) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of IRE1p in A20 cells with IRE1 alpha Polyclonal Antibody (Product # PA5-20190) at 10 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunocytochemistry of IRE1p in A20 cells with IRE1 alpha Polyclonal Antibody (Product # PA5-20190) at 1 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunofluorescence of IRE1p in A20 cells with IRE1 alpha Polyclonal Antibody (Product # PA5-20190) at 10 µg/mL.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 7 Effects of administration of metformin and PA on the major components of unfolded protein response system--PERK, ATF6, and IRE1. Immunoblotting analysis of PERK, ATF6, and IRE1 in rat VMH: the bar graphs of (a) PERK, (c) ATF6, and (e) IRE1 protein content are presented; (f) representative immunoblots are shown, and data are quantified using tubulin as a loading control for hypothalamus lysates. Quantitative RT-PCR of (b) Perk and (d) Atf6 in rat VMH: data were normalized to beta -actin and pooled from three independent experiments ( n = 6 rats/group). All data are shown as means +- SEM; * p < 0.05 vs. control, ** p < 0.05 vs. T2DM, # p < 0.05 vs. metformin administration, and @ p < 0.05 vs. PA administration.
