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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [0]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
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- Product number
- PA5-29800 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- IRE1 alpha Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: 293T, A431, HeLa, HepG2. Predicted reactivity: Mouse (99%), Rat (98%), Zebrafish (83%), Japanese Medaka (86%). Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
- Reactivity
- Human, Chicken/Avian
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 0.5 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IRE1 alpha antibody detects IRE1 alpha protein by western blot analysis. Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with IRE1 alpha antibody IRE1 alpha Polyclonal Antibody (Product # PA5-29800) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- IRE1 alpha Polyclonal Antibody detects IRE1 alpha protein at cytoplasm by immunohistochemical analysis. Sample: Paraffin-embedded rat brain. IRE1 alpha stained by IRE1 alpha Polyclonal Antibody (Product # PA5-29800) diluted at 1:500. Antigen Retrieval: Citrate buffer, pH 6.0, 15 min.
