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Western blot
ImmunocytochemistryAntibody data
- Antibody Data
- Antigen structure
- References [32]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [3]
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- Product number
- NB100-2324 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-2324, RRID:AB_10000972
- Product name
- Rabbit Polyclonal IRE1 alpha Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.1 ml
- Concentration
- 1 mg/ml
- Storage
- Store at -20C. Avoid freeze-thaw cycles.
Submitted references Molecular changes in hepatic metabolism in ZDSD rats-A new polygenic rodent model of obesity, metabolic syndrome, and diabetes.
Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation.
Guanabenz Attenuates Acetaminophen-Induced Liver Toxicity and Synergizes Analgesia in Mice.
A vascular smooth muscle cell X-box binding protein 1 and transglutaminase 2 regulatory circuit limits neointimal hyperplasia.
The IRE1α Arm of UPR Regulates Muscle Cells Immune Characters by Restraining p38 MAPK Activation.
Linking Dysregulated AMPK Signaling and ER Stress in Ethanol-Induced Liver Injury in Hepatic Alcohol Dehydrogenase Deficient Deer Mice.
Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.
Traffic-related particulate matter exposure induces nephrotoxicity in vitro and in vivo.
Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome.
Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin.
Ethanol Exposure Impairs AMPK Signaling and Phagocytosis in Human Alveolar Macrophages: Role of Ethanol Metabolism.
Liraglutide Treatment Reduces Endothelial Endoplasmic Reticulum Stress and Insulin Resistance in Patients With Diabetes Mellitus.
Inhibition of endoplasmic reticulum stress in high-fat-diet-induced obese C57BL/6 mice: Efficacy of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris.
IRE1α aggravates ischemia reperfusion injury of fatty liver by regulating phenotypic transformation of kupffer cells.
The IRE1α-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARγ activity.
Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population.
Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, endoplasmic reticulum stress, and lipogenesis and is regulated by hepatocyte nuclear factor 4 alpha in mice.
Effect of Cudrania tricuspidata and Kaempferol in Endoplasmic Reticulum Stress-Induced Inflammation and Hepatic Insulin Resistance in HepG2 Cells.
Requirement of clusterin expression for prosurvival autophagy in hypoxic kidney tubular epithelial cells.
Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation.
Elevated Endoplasmic Reticulum Stress Response Contributes to Adipose Tissue Inflammation in Aging.
Alzheimer-associated Aβ oligomers impact the central nervous system to induce peripheral metabolic deregulation.
Melatonin inhibits tunicamycin-induced endoplasmic reticulum stress and insulin resistance in skeletal muscle cells.
Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis.
The resveratrol attenuates ethanol-induced hepatocyte apoptosis via inhibiting ER-related caspase-12 activation and PDE activity in vitro.
RNF13, a RING finger protein, mediates endoplasmic reticulum stress-induced apoptosis through the inositol-requiring enzyme (IRE1α)/c-Jun NH2-terminal kinase pathway.
Carbon monoxide-releasing molecules reverse leptin resistance induced by endoplasmic reticulum stress.
Anti-apoptotic function of Xbp1 as an IL-3 signaling molecule in hematopoietic cells.
AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response.
Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1.
WFS1 is a novel component of the unfolded protein response and maintains homeostasis of the endoplasmic reticulum in pancreatic beta-cells.
Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation.
Han L, Bittner S, Dong D, Cortez Y, Bittner A, Chan J, Umar M, Shen WJ, Peterson RG, Kraemer FB, Azhar S
Biochimica et biophysica acta. Molecular basis of disease 2020 May 1;1866(5):165688
Biochimica et biophysica acta. Molecular basis of disease 2020 May 1;1866(5):165688
Interplay between Endoplasmic Reticulum (ER) Stress and Autophagy Induces Mutant p53H273 Degradation.
Garufi A, Federici G, Gilardini Montani MS, Crispini A, Cirone M, D'Orazi G
Biomolecules 2020 Mar 3;10(3)
Biomolecules 2020 Mar 3;10(3)
Guanabenz Attenuates Acetaminophen-Induced Liver Toxicity and Synergizes Analgesia in Mice.
Xie W, Xie J, Vince R, More SS
Chemical research in toxicology 2020 Jan 21;33(1):162-171
Chemical research in toxicology 2020 Jan 21;33(1):162-171
A vascular smooth muscle cell X-box binding protein 1 and transglutaminase 2 regulatory circuit limits neointimal hyperplasia.
Serrano RL, Yu W, Graham RM, Bryan RL, Terkeltaub R
PloS one 2019;14(4):e0212235
PloS one 2019;14(4):e0212235
The IRE1α Arm of UPR Regulates Muscle Cells Immune Characters by Restraining p38 MAPK Activation.
Gu R, Huang T, Xiao J, Liao Z, Li J, Lan H, Ouyang J, Hu J, Liao H
Frontiers in physiology 2019;10:1198
Frontiers in physiology 2019;10:1198
Linking Dysregulated AMPK Signaling and ER Stress in Ethanol-Induced Liver Injury in Hepatic Alcohol Dehydrogenase Deficient Deer Mice.
Srinivasan MP, Bhopale KK, Amer SM, Wan J, Kaphalia L, Ansari GS, Kaphalia BS
Biomolecules 2019 Oct 2;9(10)
Biomolecules 2019 Oct 2;9(10)
Oxytocin Suppresses Inflammatory Responses Induced by Lipopolysaccharide through Inhibition of the eIF-2-ATF4 Pathway in Mouse Microglia.
Inoue T, Yamakage H, Tanaka M, Kusakabe T, Shimatsu A, Satoh-Asahara N
Cells 2019 May 31;8(6)
Cells 2019 May 31;8(6)
Traffic-related particulate matter exposure induces nephrotoxicity in vitro and in vivo.
Hsu YH, Chuang HC, Lee YH, Lin YF, Chen YJ, Hsiao TC, Wu MY, Chiu HW
Free radical biology & medicine 2019 May 1;135:235-244
Free radical biology & medicine 2019 May 1;135:235-244
Discovery of endoplasmic reticulum calcium stabilizers to rescue ER-stressed podocytes in nephrotic syndrome.
Park SJ, Kim Y, Yang SM, Henderson MJ, Yang W, Lindahl M, Urano F, Chen YM
Proceedings of the National Academy of Sciences of the United States of America 2019 Jul 9;116(28):14154-14163
Proceedings of the National Academy of Sciences of the United States of America 2019 Jul 9;116(28):14154-14163
Characterization of the role of TMEM45A in cancer cell sensitivity to cisplatin.
Schmit K, Chen JW, Ayama-Canden S, Fransolet M, Finet L, Demazy C, D'Hondt L, Graux C, Michiels C
Cell death & disease 2019 Dec 4;10(12):919
Cell death & disease 2019 Dec 4;10(12):919
Ethanol Exposure Impairs AMPK Signaling and Phagocytosis in Human Alveolar Macrophages: Role of Ethanol Metabolism.
Kaphalia L, Srinivasan MP, Kakumanu RD, Kaphalia BS, Calhoun WJ
Alcoholism, clinical and experimental research 2019 Aug;43(8):1682-1694
Alcoholism, clinical and experimental research 2019 Aug;43(8):1682-1694
Liraglutide Treatment Reduces Endothelial Endoplasmic Reticulum Stress and Insulin Resistance in Patients With Diabetes Mellitus.
Bretón-Romero R, Weisbrod RM, Feng B, Holbrook M, Ko D, Stathos MM, Zhang JY, Fetterman JL, Hamburg NM
Journal of the American Heart Association 2018 Sep 18;7(18):e009379
Journal of the American Heart Association 2018 Sep 18;7(18):e009379
Inhibition of endoplasmic reticulum stress in high-fat-diet-induced obese C57BL/6 mice: Efficacy of a novel extract from mulberry (Morus alba) leaves fermented with Cordyceps militaris.
Lee MR, Bae SJ, Kim JE, Song BR, Choi JY, Park JJ, Park JW, Kang MJ, Choi HJ, Choi YW, Kim KM, Hwang DY
Laboratory animal research 2018 Dec;34(4):288-294
Laboratory animal research 2018 Dec;34(4):288-294
IRE1α aggravates ischemia reperfusion injury of fatty liver by regulating phenotypic transformation of kupffer cells.
Yang F, Wang S, Liu Y, Zhou Y, Shang L, Feng M, Yuan X, Zhu W, Shi X
Free radical biology & medicine 2018 Aug 20;124:395-407
Free radical biology & medicine 2018 Aug 20;124:395-407
The IRE1α-XBP1s pathway promotes insulin-stimulated glucose uptake in adipocytes by increasing PPARγ activity.
Cho YM, Kim DH, Lee KH, Jeong SW, Kwon OJ
Experimental & molecular medicine 2018 Aug 15;50(8):102
Experimental & molecular medicine 2018 Aug 15;50(8):102
Effects of a Sublethal and Transient Stress of the Endoplasmic Reticulum on the Mitochondrial Population.
Vannuvel K, Van Steenbrugge M, Demazy C, Ninane N, Fattaccioli A, Fransolet M, Renard P, Raes M, Arnould T
Journal of cellular physiology 2016 Sep;231(9):1913-31
Journal of cellular physiology 2016 Sep;231(9):1913-31
Carboxylesterase 2 prevents liver steatosis by modulating lipolysis, endoplasmic reticulum stress, and lipogenesis and is regulated by hepatocyte nuclear factor 4 alpha in mice.
Li Y, Zalzala M, Jadhav K, Xu Y, Kasumov T, Yin L, Zhang Y
Hepatology (Baltimore, Md.) 2016 Jun;63(6):1860-74
Hepatology (Baltimore, Md.) 2016 Jun;63(6):1860-74
Effect of Cudrania tricuspidata and Kaempferol in Endoplasmic Reticulum Stress-Induced Inflammation and Hepatic Insulin Resistance in HepG2 Cells.
Kim OK, Jun W, Lee J
Nutrients 2016 Jan 21;8(1)
Nutrients 2016 Jan 21;8(1)
Requirement of clusterin expression for prosurvival autophagy in hypoxic kidney tubular epithelial cells.
Alnasser HA, Guan Q, Zhang F, Gleave ME, Nguan CY, Du C
American journal of physiology. Renal physiology 2016 Jan 15;310(2):F160-73
American journal of physiology. Renal physiology 2016 Jan 15;310(2):F160-73
Hyperhomocysteinemia causes ER stress and impaired autophagy that is reversed by Vitamin B supplementation.
Tripathi M, Zhang CW, Singh BK, Sinha RA, Moe KT, DeSilva DA, Yen PM
Cell death & disease 2016 Dec 8;7(12):e2513
Cell death & disease 2016 Dec 8;7(12):e2513
Elevated Endoplasmic Reticulum Stress Response Contributes to Adipose Tissue Inflammation in Aging.
Ghosh AK, Garg SK, Mau T, O'Brien M, Liu J, Yung R
The journals of gerontology. Series A, Biological sciences and medical sciences 2015 Nov;70(11):1320-9
The journals of gerontology. Series A, Biological sciences and medical sciences 2015 Nov;70(11):1320-9
Alzheimer-associated Aβ oligomers impact the central nervous system to induce peripheral metabolic deregulation.
Clarke JR, Lyra E Silva NM, Figueiredo CP, Frozza RL, Ledo JH, Beckman D, Katashima CK, Razolli D, Carvalho BM, Frazão R, Silveira MA, Ribeiro FC, Bomfim TR, Neves FS, Klein WL, Medeiros R, LaFerla FM, Carvalheira JB, Saad MJ, Munoz DP, Velloso LA, Ferreira ST, De Felice FG
EMBO molecular medicine 2015 Feb;7(2):190-210
EMBO molecular medicine 2015 Feb;7(2):190-210
Melatonin inhibits tunicamycin-induced endoplasmic reticulum stress and insulin resistance in skeletal muscle cells.
Quan X, Wang J, Liang C, Zheng H, Zhang L
Biochemical and biophysical research communications 2015 Aug 7;463(4):1102-7
Biochemical and biophysical research communications 2015 Aug 7;463(4):1102-7
Deficiency of osteoblastic Arl6ip5 impaired osteoblast differentiation and enhanced osteoclastogenesis via disturbance of ER calcium homeostasis and induction of ER stress-mediated apoptosis.
Wu Y, Yang M, Fan J, Peng Y, Deng L, Ding Y, Yang R, Zhou J, Miao D, Fu Q
Cell death & disease 2014 Oct 16;5:e1464
Cell death & disease 2014 Oct 16;5:e1464
The resveratrol attenuates ethanol-induced hepatocyte apoptosis via inhibiting ER-related caspase-12 activation and PDE activity in vitro.
Liu LQ, Fan ZQ, Tang YF, Ke ZJ
Alcoholism, clinical and experimental research 2014 Mar;38(3):683-93
Alcoholism, clinical and experimental research 2014 Mar;38(3):683-93
RNF13, a RING finger protein, mediates endoplasmic reticulum stress-induced apoptosis through the inositol-requiring enzyme (IRE1α)/c-Jun NH2-terminal kinase pathway.
Arshad M, Ye Z, Gu X, Wong CK, Liu Y, Li D, Zhou L, Zhang Y, Bay WP, Yu VC, Li P
The Journal of biological chemistry 2013 Mar 22;288(12):8726-36
The Journal of biological chemistry 2013 Mar 22;288(12):8726-36
Carbon monoxide-releasing molecules reverse leptin resistance induced by endoplasmic reticulum stress.
Zheng M, Zhang Q, Joe Y, Kim SK, Uddin MJ, Rhew H, Kim T, Ryter SW, Chung HT
American journal of physiology. Endocrinology and metabolism 2013 Apr 1;304(7):E780-8
American journal of physiology. Endocrinology and metabolism 2013 Apr 1;304(7):E780-8
Anti-apoptotic function of Xbp1 as an IL-3 signaling molecule in hematopoietic cells.
Kurata M, Yamazaki Y, Kanno Y, Ishibashi S, Takahara T, Kitagawa M, Nakamura T
Cell death & disease 2011 Feb 10;2:e118
Cell death & disease 2011 Feb 10;2:e118
AIP1 is critical in transducing IRE1-mediated endoplasmic reticulum stress response.
Luo D, He Y, Zhang H, Yu L, Chen H, Xu Z, Tang S, Urano F, Min W
The Journal of biological chemistry 2008 May 2;283(18):11905-12
The Journal of biological chemistry 2008 May 2;283(18):11905-12
Regulation of insulin biosynthesis in pancreatic beta cells by an endoplasmic reticulum-resident protein kinase IRE1.
Lipson KL, Fonseca SG, Ishigaki S, Nguyen LX, Foss E, Bortell R, Rossini AA, Urano F
Cell metabolism 2006 Sep;4(3):245-54
Cell metabolism 2006 Sep;4(3):245-54
WFS1 is a novel component of the unfolded protein response and maintains homeostasis of the endoplasmic reticulum in pancreatic beta-cells.
Fonseca SG, Fukuma M, Lipson KL, Nguyen LX, Allen JR, Oka Y, Urano F
The Journal of biological chemistry 2005 Nov 25;280(47):39609-15
The Journal of biological chemistry 2005 Nov 25;280(47):39609-15
Fluoride induces endoplasmic reticulum stress in ameloblasts responsible for dental enamel formation.
Kubota K, Lee DH, Tsuchiya M, Young CS, Everett ET, Martinez-Mier EA, Snead ML, Nguyen L, Urano F, Bartlett JD
The Journal of biological chemistry 2005 Jun 17;280(24):23194-202
The Journal of biological chemistry 2005 Jun 17;280(24):23194-202
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRE1 alpha Antibody [NB100-2324] - Total IRE1 Alpha protein in lysates from Min6 cells which were transfected with GFP-siRNA or Ire1-siRNA. Theoretical molecular weight: 110 kDa.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: IRE1 alpha Antibody [NB100-2324] - ALOX15 knockout blocks alcohol-induced up-regulation of IRE1a in mouse liver. Image from verified customer review.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NB100-2324] - Analysis of a formalin fixed mouse colon tissue section using total IRE1 alpha antibody (NB100-2324) at 1:300. Note a strong cytoplasmic signal in various cells of colonic villi (columnar epithelial and goblet cells) and the muscular layer. A few cells, potentially with active UPR, showed nuclear positivity also while the signal was negligible in cells at base of crypts and lamina propria.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: IRE1 alpha Antibody [NB100-2324] - Analysis of a formalin fixed mouse colon tissue section using total IRE1 alpha antibody (NB100-2324) at 1:300. Note a strong cytoplasmic signal in various cells of colonic villi (columnar epithelial and goblet cells) and the muscular layer. Some cells, most likely with active UPR, depicted nuclear positivity also while the signal was negligible in crypts cells and lamina propria.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry: IRE1 alpha Antibody [NB100-2324] - XBP1 and TG2 deficiency promote increased IRE1a protein levels in cultured VSMCs and in neointima in situ. (D) Xbp1WT and Xbp1 delta WT and (E) Tgm2 WT and Tgm2 delta carotid artery sections were analyzed by IHC for total IRE1a. Image collected and cropped by CiteAb from the following publication (//doi.org/10.1371/journal.pone.0212235) licensed under a CC-BY licence.
