LS-C355507

antibody from LSBio

Targeting: FURIN FUR, PACE, PCSK3, SPC1

Western blot (Supportive data in Antibodypedia)

Immunocytochemistry (Data presented on provider website)

Immunoprecipitation (Recommended by provider)

Antibody Data

Product number

LS-C355507

Provider

LSBio

Product name

FURIN Antibody LS-C355507

Antibody type

Polyclonal

Description

Immunoaffinity purified

Reactivity

Human, Mouse, Rat, Canine, Hamster, Horse, Sheep, Simian

Host

Rabbit

Isotype

IgG

Storage

Store at -20°C. Avoid freeze-thaw cycles.

Western blot

Supportive validation

Submitted by

LSBio (provider)

Main image

Experimental details

Western blot analysis was performed on membrane enriched extracts (30 µg lysate) of HeLa (Lane 1), HEK 293 (Lane 2), K-562 (Lane 3), SH-SY5Y (Lane 4) and 10uL conditioned media from HeLa cell line (Lane 5). The blot was probed with Anti-Furin Convertase Rabbit Polyclonal Antibody and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal Secondary Antibody, HRP conjugate. A 87 kDa band corresponding to Furin Convertase was observed across the cell lines and conditioned media tested. Known quantity of protein samples were electrophoresed using 10 % Bis-Tris gel. Resolved proteins were then transferred onto a nitrocellulose membrane. The membrane was probed with the relevant primary and secondary Antibody using iBind Flex Western Starter Kit.