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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [7]
- Immunohistochemistry [2]
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Validation data
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- Product number
- PA5-27281 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- RPSA Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant protein fragment
- Description
- Recommended positive controls: HepG2, Jurkat, Mouse brain.
- Concentration
- 0.1 mg/mL
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of RPSA using 30 µg of HepG2 lysate. Samples were loaded onto a 12% SDS-PAGE gel and probed with a RPSA polyclonal antibody (Product # PA5-27281) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPSA was performed by separating 30 µg of Jurkat lysates by 12% SDS PAGE. Proteins were transferred to a membrane and probed with a RPSA Polyclonal Antibody (Product # PA5-27281) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPSA Polyclonal Antibody (Product # PA5-27281). Sample (50 µg of whole cell lysate). Lane A: mouse brain. 12% SDS PAGE. RPSA Polyclonal Antibody (Product # PA5-27281) diluted at 1:1,000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot using RPSA Polyclonal Antibody (Product # PA5-27281). Various whole cell extracts (30 µg) were separated by 12% SDS-PAGE, and the membrane was blotted with RPSA Polyclonal Antibody (Product # PA5-27281) diluted at 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot analysis of RPSA was performed by separating 30 µg of whole cell extracts by 10% SDS-PAGE. Proteins were transferred to a membrane and probed with a RPSA Polyclonal Antibody (Product # PA5-27281) at a dilution of 1:1000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPSA antibody detects RPSA protein by Western blot analysis. Mouse tissue extracts (50 µg) was separated by 10 % SDS-PAGE, and the membrane was blotted with RPSA antibody RPSA Polyclonal Antibody (Product # PA5-27281) diluted by 1:1,000.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPSA antibody detects RPSA protein by Western blot analysis. Whole cell extracts (30 µg) was separated by 10 % SDS-PAGE, and blotted with RPSA antibody RPSA Polyclonal Antibody (Product # PA5-27281) diluted by 1:1,000.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of paraffin-embedded CA922 xenograft, using RPSA (Product # PA5-27281) antibody at 1:500 dilution. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- RPSA Polyclonal Antibody detects RPSA protein at cytoplasm on mouse small intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse small intestine. RPSA Polyclonal Antibody (Product # PA5-27281) diluted at 1:500. Antigen Retrieval: EDTA based buffer, pH 8.0, 15 min.
