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Western blot
Immunocytochemistry
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [12]
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- Validations
- Western blot [1]
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- Product number
- PA5-16581 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Anti-GCLC Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Synthetic peptide
- Description
- PA5-16581 targets Gamma-Glutamyl cysteine Synthetase/Glutamate-cysteine Ligase in IP and WB applications and shows reactivity with Bovine, Human, mouse, Non-human primate, and Rat samples. The PA5-16581 immunogen is a synthetic peptide derived from corresponding to aa 295-313 of rat GCLC (heavy subunit of GCS).
- Reactivity
- Human, Mouse, Rat, Bovine
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 500 µL
- Concentration
- 1 mg/mL
- Storage
- 4° C
Submitted references Protection against oxidative stress mediated by the Nrf2/Keap1 axis is impaired in Primary Biliary Cholangitis.
Does hepatic oxidative stress enhance activation of nuclear factor-E2-related factor in patients with nonalcoholic steatohepatitis?
Alterations in the metabolomics of sulfur-containing substances in rat kidney by betaine.
Impaired transcriptional activity of Nrf2 in age-related myocardial oxidative stress is reversible by moderate exercise training.
Disruption of Nrf2/ARE signaling impairs antioxidant mechanisms and promotes cell degradation pathways in aged skeletal muscle.
Acute exercise stress activates Nrf2/ARE signaling and promotes antioxidant mechanisms in the myocardium.
Microtubules as a critical target for arsenic toxicity in lung cells in vitro and in vivo.
Isolation, identification, and biological evaluation of Nrf2-ARE activator from the leaves of green perilla (Perilla frutescens var. crispa f. viridis).
Cross-regulations among NRFs and KEAP1 and effects of their silencing on arsenic-induced antioxidant response and cytotoxicity in human keratinocytes.
Increased glutathione synthesis following Nrf2 activation by vanadyl sulfate in human chang liver cells.
Carbon monoxide produced by heme oxygenase-1 in response to nitrosative stress induces expression of glutamate-cysteine ligase in PC12 cells via activation of phosphatidylinositol 3-kinase and Nrf2 signaling.
Expression of antioxidant enzymes in rat retinal ischemia followed by reperfusion.
Wasik U, Milkiewicz M, Kempinska-Podhorodecka A, Milkiewicz P
Scientific reports 2017 Mar 23;7:44769
Scientific reports 2017 Mar 23;7:44769
Does hepatic oxidative stress enhance activation of nuclear factor-E2-related factor in patients with nonalcoholic steatohepatitis?
Takahashi Y, Kobayashi Y, Kawata K, Kawamura K, Sumiyoshi S, Noritake H, Watanabe S, Chida T, Souda K, Sakaguchi T, Nakamura H, Suda T
Antioxidants & redox signaling 2014 Jan 20;20(3):538-43
Antioxidants & redox signaling 2014 Jan 20;20(3):538-43
Alterations in the metabolomics of sulfur-containing substances in rat kidney by betaine.
Kim YC, Kwon DY, Kim JH
Amino acids 2014 Apr;46(4):963-8
Amino acids 2014 Apr;46(4):963-8
Impaired transcriptional activity of Nrf2 in age-related myocardial oxidative stress is reversible by moderate exercise training.
Gounder SS, Kannan S, Devadoss D, Miller CJ, Whitehead KJ, Odelberg SJ, Firpo MA, Paine R 3rd, Hoidal JR, Abel ED, Rajasekaran NS
PloS one 2012;7(9):e45697
PloS one 2012;7(9):e45697
Disruption of Nrf2/ARE signaling impairs antioxidant mechanisms and promotes cell degradation pathways in aged skeletal muscle.
Miller CJ, Gounder SS, Kannan S, Goutam K, Muthusamy VR, Firpo MA, Symons JD, Paine R 3rd, Hoidal JR, Rajasekaran NS
Biochimica et biophysica acta 2012 Jun;1822(6):1038-50
Biochimica et biophysica acta 2012 Jun;1822(6):1038-50
Acute exercise stress activates Nrf2/ARE signaling and promotes antioxidant mechanisms in the myocardium.
Muthusamy VR, Kannan S, Sadhaasivam K, Gounder SS, Davidson CJ, Boeheme C, Hoidal JR, Wang L, Rajasekaran NS
Free radical biology & medicine 2012 Jan 15;52(2):366-76
Free radical biology & medicine 2012 Jan 15;52(2):366-76
Microtubules as a critical target for arsenic toxicity in lung cells in vitro and in vivo.
Zhao Y, Toselli P, Li W
International journal of environmental research and public health 2012 Feb;9(2):474-95
International journal of environmental research and public health 2012 Feb;9(2):474-95
Isolation, identification, and biological evaluation of Nrf2-ARE activator from the leaves of green perilla (Perilla frutescens var. crispa f. viridis).
Izumi Y, Matsumura A, Wakita S, Akagi K, Fukuda H, Kume T, Irie K, Takada-Takatori Y, Sugimoto H, Hashimoto T, Akaike A
Free radical biology & medicine 2012 Aug 15;53(4):669-79
Free radical biology & medicine 2012 Aug 15;53(4):669-79
Cross-regulations among NRFs and KEAP1 and effects of their silencing on arsenic-induced antioxidant response and cytotoxicity in human keratinocytes.
Zhao R, Hou Y, Zhang Q, Woods CG, Xue P, Fu J, Yarborough K, Guan D, Andersen ME, Pi J
Environmental health perspectives 2012 Apr;120(4):583-9
Environmental health perspectives 2012 Apr;120(4):583-9
Increased glutathione synthesis following Nrf2 activation by vanadyl sulfate in human chang liver cells.
Kim AD, Zhang R, Kang KA, You HJ, Hyun JW
International journal of molecular sciences 2011;12(12):8878-94
International journal of molecular sciences 2011;12(12):8878-94
Carbon monoxide produced by heme oxygenase-1 in response to nitrosative stress induces expression of glutamate-cysteine ligase in PC12 cells via activation of phosphatidylinositol 3-kinase and Nrf2 signaling.
Li MH, Jang JH, Na HK, Cha YN, Surh YJ
The Journal of biological chemistry 2007 Sep 28;282(39):28577-86
The Journal of biological chemistry 2007 Sep 28;282(39):28577-86
Expression of antioxidant enzymes in rat retinal ischemia followed by reperfusion.
Agardh CD, Gustavsson C, Hagert P, Nilsson M, Agardh E
Metabolism: clinical and experimental 2006 Jul;55(7):892-8
Metabolism: clinical and experimental 2006 Jul;55(7):892-8
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Supportive validation
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- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot of Gamma-Glutamyl cysteine Synthetase/Glutamate-cysteine Ligase using Gamma-Glutamyl cysteine Synthetase/Glutamate-cysteine Ligase Polyclonal Antibody (Product # PA5-16581) on Jurkat Cells.
