15520-1-AP

antibody from Proteintech Group

Targeting: MSH2 COCA1, HNPCC, HNPCC1

Provider product page for 15520-1-AP

Western blot

ELISA

Immunocytochemistry

Immunoprecipitation

Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [27]
Comments [0]
Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

Submit

Product number: 15520-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15520-1-AP, RRID:AB_2144799
Product name: MSH2 antibody
Antibody type: Polyclonal
Description: KD/KO validated MSH2 antibody (Cat. #15520-1-AP) is a rabbit polyclonal antibody that shows reactivity with human and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

MSH2 protein structure - 15520-1-AP shown in red.

Submitted references Intercellular adhesion molecule-1 suppresses TMZ chemosensitivity in acquired TMZ-resistant gliomas by increasing assembly of ABCB1 on the membrane.

Zhang X, Tan Y, Li T, Tan D, Fu B, Yang M, Chen Y, Cao M, Xuan C, Du Q, Hu R, Wang Q
Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy 2024 Sep;76:101112

MRE11A: a novel negative regulator of human DNA mismatch repair.

Du D, Yang Y, Zhang Y, Wang G, Chen L, Guan X, Rasmussen LJ, Liu D
Cellular & molecular biology letters 2024 Mar 14;29(1):37

Nicotinamide metabolism face-off between macrophages and fibroblasts manipulates the microenvironment in gastric cancer.

Jiang Y, Wang Y, Chen G, Sun F, Wu Q, Huang Q, Zeng D, Qiu W, Wang J, Yao Z, Liang B, Li S, Wu J, Huang N, Wang Y, Chen J, Zhai X, Huang L, Xu B, Yamamoto M, Tsukamoto T, Nomura S, Liao W, Shi M
Cell metabolism 2024 Aug 6;36(8):1806-1822.e11

The MSH2 c.793-1G>A variant disrupts normal splicing and is associated with Lynch syndrome.

Li Y, Yu L, Cui J, Yin J, Wu W
Frontiers in oncology 2023;13:1131011

Lnc-PTCHD4-AS inhibits gastric cancer through MSH2-MSH6 dimerization and ATM-p53-p21 activation.

Wang J, Mi Y, Sun X, Xue X, Zhao H, Zhang M, Hu B, Bukhari I, Zheng P
Aging 2023 Nov 27;15(22):13558-13578

Targeting DNA polymerase β elicits synthetic lethality with mismatch repair deficiency in acute lymphoblastic leukemia.

Teng JY, Yang DP, Tang C, Fang HS, Sun HY, Xiang YN, Li XM, Yang F, Xia RX, Fan F, Liu J, Yu J, Hu JC, Li BS, Li H, Meng FL, Duan CW, Zhou BS
Leukemia 2023 Jun;37(6):1204-1215

NAT10 mediated mRNA acetylation modification patterns associated with colon cancer progression and microsatellite status.

Zhang H, Shan W, Yang Z, Zhang Y, Wang M, Gao L, Zeng L, Zhao Q, Liu J
Epigenetics 2023 Dec;18(1):2188667

MiR-590-5p promotes cisplatin resistance via targeting hMSH2 in ovarian cancer.

Li B, Xu X, Zheng L, Jiang X, Lin J, Zhang G
Molecular biology reports 2023 Aug;50(8):6819-6827

Loss of TDP-43 promotes somatic CAG repeat expansion in Huntington's disease knock-in mice.

Bai D, Zhu L, Jia Q, Duan X, Chen L, Wang X, Hou J, Jiang G, Yang S, Li S, Li XJ, Yin P
Progress in neurobiology 2023 Aug;227:102484

Molecular Classification in Patients With Endometrial Cancer After Fertility-Preserving Treatment: Application of ProMisE Classifier and Combination of Prognostic Evidence.

Ran X, Hu T, Li Z
Frontiers in oncology 2022;12:810631

In Situ Peroxidase Labeling Followed by Mass-Spectrometry Reveals TIA1 Interactome.

Gourdomichali O, Zonke K, Kattan FG, Makridakis M, Kontostathi G, Vlahou A, Doxakis E
Biology 2022 Feb 11;11(2)

Lack of association of somatic CAG repeat expansion with striatal neurodegeneration in HD knock-in animal models.

Bai D, Yin P, Zhang Y, Sun F, Chen L, Lin L, Yan S, Li S, Li XJ
Human molecular genetics 2021 Jul 28;30(16):1497-1508

Nrf2 overexpression increases risk of high tumor mutation burden in acute myeloid leukemia by inhibiting MSH2.

Liu P, Ma D, Wang P, Pan C, Fang Q, Wang J
Cell death & disease 2021 Jan 5;12(1):20

Chemotherapy and mismatch repair deficiency cooperate to fuel TP53 mutagenesis and ALL relapse.

Yang F, Brady SW, Tang C, Sun H, Du L, Barz MJ, Ma X, Chen Y, Fang H, Li X, Kolekar P, Pathak O, Cai J, Ding L, Wang T, von Stackelberg A, Shen S, Eckert C, Klco JM, Chen H, Duan C, Liu Y, Li H, Li B, Kirschner-Schwabe R, Zhang J, Zhou BS
Nature cancer 2021 Aug;2(8):819-834

Clinicopathological and survival characteristic of mismatch repair status in ovarian clear cell carcinoma.

Zhu J, Ke G, Bi R, Wu X
Journal of surgical oncology 2020 Sep;122(3):538-546

KALRN mutations promote antitumor immunity and immunotherapy response in cancer.

Li M, Ma Y, Zhong Y, Liu Q, Chen C, Qiang L, Wang X
Journal for immunotherapy of cancer 2020 Oct;8(2)

m(6) A RNA methyltransferases METTL3/14 regulate immune responses to anti-PD-1 therapy.

Wang L, Hui H, Agrawal K, Kang Y, Li N, Tang R, Yuan J, Rana TM
The EMBO journal 2020 Oct 15;39(20):e104514

Quantitative Proteomics Analysis Reveals Nuclear Perturbation in Human Glioma U87 Cells treated with Temozolomide.

Guo J, Yi GZ, Liu Z, Sun X, Yang R, Guo M, Li Y, Li K, Li K, Wang X, Song H, Qi S, Huang G, Liu Y
Cell biochemistry and function 2020 Mar;38(2):185-194

Aberrantly expressed HORMAD1 disrupts nuclear localization of MCM8-MCM9 complex and compromises DNA mismatch repair in cancer cells.

Liu K, Wang Y, Zhu Q, Li P, Chen J, Tang Z, Shen Y, Cheng X, Lu LY, Liu Y
Cell death & disease 2020 Jul 9;11(7):519

Mismatch repair status as a beneficial predictor of fluorouracil-based adjuvant chemotherapy for pancreatic cancer.

Liang D, Shi S, Liang C, Meng Q, Zhang B, Ni Q, Xu J, Yu X
Surgery 2018 May;163(5):1080-1089

A highly malignant case of neuroblastoma with substantial increase of single-nucleotide variants and normal mismatch repair system: A case report.

Yuan LQ, Wang JH, Zhu K, Yang M, Gu WZ, Lai C, Li HM, Shu Q, Chen X
Medicine 2017 Dec;96(50):e8845

Significance of p53 expression in background endometrium in endometrial carcinoma.

Nguyen TT, Hachisuga T, Urabe R, Kurita T, Kagami S, Kawagoe T, Shimajiri S, Nabeshima K
Virchows Archiv : an international journal of pathology 2015 Jun;466(6):695-702

Differential mucin phenotypes and their significance in a variation of colorectal carcinoma.

Imai Y, Yamagishi H, Fukuda K, Ono Y, Inoue T, Ueda Y
World journal of gastroenterology 2013 Jul 7;19(25):3957-68

Aberrant cytokeratin expression as a possible prognostic predictor in poorly differentiated colorectal carcinoma.

Yamagishi H, Imai Y, Okamura T, Fukuda K, Ono Y, Ban S, Inoue T, Ueda Y
Journal of gastroenterology and hepatology 2013 Dec;28(12):1815-22

Ectopic expression of human MutS homologue 2 on renal carcinoma cells is induced by oxidative stress with interleukin-18 promotion via p38 mitogen-activated protein kinase (MAPK) and c-Jun N-terminal kinase (JNK) signaling pathways.

Mo C, Dai Y, Kang N, Cui L, He W
The Journal of biological chemistry 2012 Jun 1;287(23):19242-54

Estradiol regulates miR-135b and mismatch repair gene expressions via estrogen receptor-β in colorectal cells.

He YQ, Sheng JQ, Ling XL, Fu L, Jin P, Yen L, Rao J
Experimental & molecular medicine 2012 Dec 31;44(12):723-32

Estrogen stimulates the expression of mismatch repair gene hMLH1 in colonic epithelial cells.

Jin P, Lu XJ, Sheng JQ, Fu L, Meng XM, Wang X, Shi TP, Li SR, Rao J
Cancer prevention research (Philadelphia, Pa.) 2010 Aug;3(8):910-6

No comments: Submit comment

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 15520-1-AP(MSH2 antibody) at dilution of 1:500
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using MSH2 antibody 15520-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 15520-1-AP(MSH2 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 15520-1-AP(MSH2 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue