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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [3]
- Immunocytochemistry [3]
- Immunohistochemistry [2]
- Flow cytometry [1]
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- Product number
- R31697 - Provider product page
- Provider
- NSJ Bioreagents
- Product name
- MSH2 Antibody
- Antibody type
- Polyclonal
- Description
- This highly specific MSH2 antibody is suitable for use in Western blot/Immunohistochemistry/Immunocytochemistry/Immunofluorescence/Flow cytometry applications with human, mouse and rat samples.
- Reactivity
- Human, Mouse, Rat
- Host
- Rabbit
- Conjugate
- Unconjugated
- Vial size
- 100 ug
- Concentration
- 0.5mg/ml if reconstituted with 0.2ml sterile DI water
- Storage
- After reconstitution, the MSH2 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
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Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) HEK293, 2) HEK293 (different lot), 3) HeLa, 4) COLO-320, 5) T-47D and 6) A549 cell lysate with MSH2 antibody. Expected molecular weight: ~105 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of 1) human K562, 2) human SH-SY5Y, 3) human HEL, 4) human 293T, 5) human A549, 6) rat PC-12 and 7) mouse Neuro-2a cell lysate with MSH2 antibody. Expected molecular weight: ~105 kDa.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Western blot testing of human 1) K562, 2) SH-SY5Y, 3) 293T and 4) A549 cell lysate with MSH2 antibody (DyLight 647 secondary on left and HRP secondary on right). Expected molecular weight: ~105 kDa.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of MSH2 antibody and SMMC-7721 cells
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- ICC testing of MSH2 antibody and SMMC-7721 cells
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Immunofluorescent staining of FFPE human U-2 OS cells with MSH2 antibody (green) and DAPI nuclear stain (blue). HIER: boil tissue sections in pH6, 10mM citrate buffer, for 20 min and allow to cool before testing.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MSH2 antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- IHC-P: MSH2 antibody testing of human intestine cancer tissue. HIER: steamed with pH6 citrate buffer.
Supportive validation
- Submitted by
- NSJ Bioreagents (provider)
- Main image
- Experimental details
- Flow cytometry testing of human A431 cells with MSH2 antibody at 1ug/million cells (blocked with goat sera); Red=cells alone, Green=isotype control, Blue= MSH2 antibody.
