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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
- Comments [0]
- Validations
- Western blot [2]
- Immunoprecipitation [1]
- Immunohistochemistry [2]
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- Product number
- NB100-621 - Provider product page
- Provider
- Novus Biologicals
- Proper citation
- Novus Cat#NB100-621, RRID:AB_2145123
- Product name
- Rabbit Polyclonal MSH2 Antibody
- Antibody type
- Polyclonal
- Description
- Immunogen affinity purified.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 ul
- Concentration
- 1.0 mg/ml
- Storage
- Store at 4C. Do not freeze.
Submitted references Mismatch tolerance during homologous recombination in mammalian cells.
Li S, Wehrenberg B, Waldman BC, Waldman AS
DNA repair 2018 Oct;70:25-36
DNA repair 2018 Oct;70:25-36
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MSH2 Antibody [NB100-621] - Detection of Human and Mouse MSH2 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa, 293T, and mouse NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: MSH2 Antibody [NB100-621] - Detection of human and mouse MSH2 by Western blot and immunoprecipitation using NB100-621. Detection: ECL with a 3 minute exposure.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunoprecipitation: MSH2 Antibody [NB100-621] - Detection of human MSH2 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-MSH2 antibody NB100-621 used for IP at 6 ug per reaction. MSH2 was also immunoprecipitated by another rabbit anti-MSH2 antibody. For blotting immunoprecipitated MSH2, NB100-621 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MSH2 Antibody [NB100-621] - FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-MSH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: MSH2 Antibody [NB100-621] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-MSH2 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
