H00004638-D01P

antibody from Abnova Corporation

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Western blot (Supportive data in Antibodypedia)

Proximity ligation assay (Supportive data in Antibodypedia)

Antibody Data

Product number

H00004638-D01P

Provider

Abnova Corporation

Proper citation

Abnova Corporation Cat#H00004638-D01P, RRID:AB_1577296

Product name

MYLK purified MaxPab rabbit polyclonal antibody (D01P)

Antibody type

Polyclonal

Description

Rabbit polyclonal antibody raised against a full-length human MYLK protein.

Antigen sequence

MDFRANLQRQVKPKTVSEEERKVHSPQQVDFRSVL
AKKGTSKTPVPEKVPPPKPATPDFRSVLGGKKKLP
AENGSSSAETLNAKAVESSKPLSNAQPSGPLKPVG
NAKPAETLKPMGNAKPAETLKPMGNAKPDENLKSA
SKEELKKDVKNDVNCKRGHAGTTDNEKRSESQGTA
PAFKQKLQDVHVAEGKKLLLQCQVSSDPPATIIWT
LNGKTLKTTKFIILSQEGSLCSVSIEKALPEDRGL
YKCVAKNDAGQAECSCQVTVDDAPASENTKAPEMK
SRRPKSSLPPVLGTESDATVKKKPAPKTPPKAAMP
PQIIQFPEDQKVRAGESVELFGKVTGTQPITCTWM
KFRKQIQESEHMKVENSENGSKLTILAARQEHCGC
YTLLVENKLGSRQAQVNLTVVDKPDPPAGTPCASD
IRSSSLTLSWYGSSYDGGSAVQSYSIEIWDSANKT
WKELATCRSTSFNVQDLLPDHEYKFRVRAINVYGT
SEPSQESELTTVGEKPEEPKDEVEVSDDDEKEPEV
DYRTVTINTEQKVSDFYDIEERLGSGKFGQVFRLV
EKKTRKVWAGKFFKAYSAKEKENIRQEISIMNCLH
HPKLVQCVDAFEEKANIVMVLEIVSGGELFERIID
EDFELTERECIKYMRQISEGVEYIHKQGIVHLDLK
PENIMCVNKTGTRIKLIDFGLARRLENAGSLKVLF
GTPEFVAPEVINYEPIGYATDMWSIGVICYILVSG
LSPFMGDNDNETLANVTSATWDFDDEAFDEISDDA
KDFISNLLKKDMKNRLDCTQCLQHPWLMKDTKNME
AKKLSKDRMKKYMARRKWQKTGNAVRAIGRLSSMA
MISGLSGRKSSTGSPTSPLNAEKLESEEDVSQAFL
EAVAEEKPHVKPYFSKTIRDLEVVEGSAARFDCKI
EGYPDPEVVWFKDDQSIRESRHFQIDYDEDGNCSL
IISDVCGDDDAKYTCKAVNSLGEATCTAELIVETM
EEGEGEGEEEEE


Storage

Store at -20°C or lower. Aliquot to avoid repeated freezing and thawing.

Western blot

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Western Blot analysis of MYLK expression in transfected 293T cell line (H00004638-T01) by MYLK MaxPab polyclonal antibody.Lane 1: MYLK transfected lysate(110.20 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

MYLK MaxPab rabbit polyclonal antibody. Western Blot analysis of MYLK expression in human stomach.

Proximity ligation assay

Supportive validation

Submitted by

Abnova Corporation (provider)

Main image

Experimental details

Proximity Ligation Analysis of protein-protein interactions between MYLK and CTTN. HeLa cells were stained with anti-MYLK rabbit purified polyclonal 1:1200 and anti-CTTN mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

Validation comment

In situ Proximity Ligation Assay (Cell)