PA5-15177

antibody from Invitrogen Antibodies

Targeting: MYLK MLCK, MLCK1, MYLK1, smMLCK

Western blot (Supportive data in Antibodypedia)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

PA5-15177

Provider

Invitrogen Antibodies

Product name

MYLK Polyclonal Antibody

Antibody type

Polyclonal

Antigen

Synthetic peptide

Description

This antibody is predicted to react with rabbit based on sequence homology.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

400 µL

Concentration

1.7 mg/mL

Storage

-20° C, Avoid Freeze/Thaw Cycles

References

Submitted references

Behind Brain Metastases Formation: Cellular and Molecular Alterations and Blood-Brain Barrier Disruption.

Godinho-Pereira J, Garcia AR, Figueira I, Malhó R, Brito MA
International journal of molecular sciences 2021 Jun 30;22(13)

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Picturing Breast Cancer Brain Metastasis Development to Unravel Molecular Players and Cellular Crosstalk.

Figueira I, Galego S, Custódio-Santos T, Vicente R, Molnár K, Haskó J, Malhó R, Videira M, Wilhelm I, Krizbai I, Brito MA
Cancers 2021 Feb 22;13(4)

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Listeria Adhesion Protein Induces Intestinal Epithelial Barrier Dysfunction for Bacterial Translocation.

Drolia R, Tenguria S, Durkes AC, Turner JR, Bhunia AK
Cell host & microbe 2018 Apr 11;23(4):470-484.e7

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a MLCK polyclonal antibody (Product # PA5-15177) in SK-BR-3 cell lysates (35 µg per lane).

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis using a MLCK polyclonal antibody (Product # PA5-15177) in mouse brain tissue lysate. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

NULL

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Figure 6 Interaction between breast cancer cells and brain microvascular endothelial cells leads to cytoskeleton rearrangement. Confluent monolayers of the brain endothelioma cell line b.End5 under physiological laminar non-pulsatile shear stress were exposed to 4T1 breast cancer cells (previously labelled with CellTracker(tm) DMTPX Red Dye) for 1, 3, 6 and 24 h and the expression of myosin light chain kinase (MLCK), as well as of phosphorylated myosin light chain (p-MLC), in single and mixed cultures was evaluated by immunofluorescence analysis. ( A ) Analysis of the expression of MLCK (yellow) revealed that the cytoskeleton-associated protein is present in both cell types, with a marked overexpression in b.End5 cells exposed to 4T1 cells at 6 h (as shown by the white arrows). Hoechst 33342 was used as counterstaining for nuclei (blue). Scale bar: 20 um. ( B ) Semi-quantitative analysis revealed an increase in endothelial MLCK intensity in mixed cultures, particularly at 6 h. A significant increase in ( C ) cytoplasmic and ( D ) nuclear MLCK in mixed cultures compared with single ones was observed. ( E ) Cytoskeleton rearrangements were confirmed by the increase in p-MLC (yellow) observed in the cytoplasm of b.End5 cells (circumscribed by dotted line) exposed to 4T1 cells at 6 h. Hoechst 33342 was used as counterstaining for nuclei (blue). Scale bar: 10 um. ( F ) Semi-quantitative analysis of the morphology of p-MLC stained b.End5 revealed a decrease in elongation upon incub