HPA039293

antibody from Atlas Antibodies

Targeting: SPTBN2 SCA5

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Enhanced validation)

Antibody Data

Product number

HPA039293

Provider

Atlas Antibodies

Proper citation

Atlas Antibodies Cat#HPA039293, RRID:AB_2676430

Product name

Anti-SPTBN2

Antibody type

Polyclonal

Reactivity

Human

Host

Rabbit

Conjugate

Unconjugated

Antigen sequence

PPPSTQAPSVNGVCTDGEPSQPLLGQQRLEHSSFP
EGPGPGSGDEANGPRGERQTRTRGPAPSAMPQSRS
TESAH


Isotype

IgG

Vial size

100 µl

Storage

Store at +4°C for short term storage. Long time storage is recommended at -20°C.

Immunocytochemistry

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunofluorescent staining of human cell line A-431 shows localization to cytosol.

Sample type

HUMAN

Immunohistochemistry

Enhanced validation

Submitted by

Atlas Antibodies (provider)

Enhanced method

Orthogonal validation

Main image

Experimental details

Immunohistochemistry analysis in human skin and endometrium tissues using Anti-SPTBN2 antibody. Corresponding SPTBN2 RNA-seq data are presented for the same tissues.

Sample type

HUMAN

Orthogonal method

Orthogonal validation of protein expression using IHC by comparison to RNA-seq data of corresponding target in high and low expression tissues.

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Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human cerebellum shows strong cytoplasmic positivity in Purkinje cells, granular and molecular layer cells.

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human skin shows high expression.

Sample type

HUMAN

Supportive validation

Submitted by

Atlas Antibodies (provider)

Main image

Experimental details

Immunohistochemical staining of human endometrium shows low expression as expected.

Sample type

HUMAN