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Western blot
ImmunoprecipitationAntibody data
- Antibody Data
- Antigen structure
- References [3]
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- Validations
- Western blot [1]
- Immunohistochemistry [1]
- Other assay [1]
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- Product number
- PA1-46007 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Spectrin beta-3 Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Other
- Description
- Suggested positive control: Hela whole cell extract.
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 100 µL
- Concentration
- 1 mg/mL
- Storage
- Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Submitted references MTSS1/Src family kinase dysregulation underlies multiple inherited ataxias.
A sensitive, reproducible and objective immunofluorescence analysis method of dystrophin in individual fibers in samples from patients with duchenne muscular dystrophy.
Prednisolone treatment does not interfere with 2'-O-methyl phosphorothioate antisense-mediated exon skipping in Duchenne muscular dystrophy.
Brown AS, Meera P, Altindag B, Chopra R, Perkins EM, Paul S, Scoles DR, Tarapore E, Magri J, Huang H, Jackson M, Shakkottai VG, Otis TS, Pulst SM, Atwood SX, Oro AE
Proceedings of the National Academy of Sciences of the United States of America 2018 Dec 26;115(52):E12407-E12416
Proceedings of the National Academy of Sciences of the United States of America 2018 Dec 26;115(52):E12407-E12416
A sensitive, reproducible and objective immunofluorescence analysis method of dystrophin in individual fibers in samples from patients with duchenne muscular dystrophy.
Beekman C, Sipkens JA, Testerink J, Giannakopoulos S, Kreuger D, van Deutekom JC, Campion GV, de Kimpe SJ, Lourbakos A
PloS one 2014;9(9):e107494
PloS one 2014;9(9):e107494
Prednisolone treatment does not interfere with 2'-O-methyl phosphorothioate antisense-mediated exon skipping in Duchenne muscular dystrophy.
Verhaart IE, Heemskerk H, Karnaoukh TG, Kolfschoten IG, Vroon A, van Ommen GJ, van Deutekom JC, Aartsma-Rus A
Human gene therapy 2012 Mar;23(3):262-73
Human gene therapy 2012 Mar;23(3):262-73
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western Blot detection of Human SPTBN2/SCA5 on HeLa whole cell lysates using Product # PA1-46007. SPTBN2/SCA5 was also immunoprecipitated by rabbit anti-SPTBN2/SCA5 antibody.
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Spectrin beta-3 in human prostate epithelium. Samples were incubated in Spectrin beta-3 polyclonal antibody (Product # PA1-46007).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Figure 1 Immunofluorescence analysis of dystrophin intensities per fiber in a muscle fiber population. A. Cryosections of a healthy control muscle sample (control 3, quadriceps muscle) co-stained with anti-dystrophin antibody MANDYS106 and an anti-spectrin antibody. The sections were imaged using a confocal microscope, with the green channel (Alexa488) showing dystrophin and the red channel (Alexa594) showing spectrin. B. Images were analyzed using customized Definiens software, which automatically identifies individual muscle fiber membranes using their spectrin signal and measures the membrane dystrophin intensity per muscle fiber. C. Dystrophin intensity distribution in muscle fiber population analyzed, shown as a regular histogram (blue line) or a cumulative histogram (red line). (au: arbitrary units).
