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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
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Validation data
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- Product number
- 100-401-J03 - Provider product page
- Provider
- Rockland Immunochemicals, Inc.
- Proper citation
- Rockland Cat#100-401-J03, RRID:AB_2610777
- Product name
- Anti-Myosin 6 (Rabbit) Antibody - 100-401-J03
- Antibody type
- Polyclonal
- Vial size
- 100 µl
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Supportive validation
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- Rockland Immunochemicals, Inc. (provider)
- Main image
- Experimental details
- Western Blot of Rabbit anti-Myosin-6 antibody. Lane 1: SF9 cell lysate of truncated smooth myosin. Lane 2: Jurkat lysate. Lane 3: LnCap lysate. Lane 4: Recombinant myosin VI Load: 35 µg per lane for cell lysate. 50 ng of recombinant protein. Primary antibody: Myosin 6 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 150 kDa for Myo6.
- Validation comment
- Western Blot
