100-401-J04S

antibody from Rockland Immunochemicals, Inc.

Targeting: KIF5B KNS, KNS1

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

100-401-J04S

Provider

Rockland Immunochemicals, Inc.

Proper citation

Rockland Cat#100-401-J04S, RRID:AB_2610780

Product name

Anti-Eg5 (RABBIT) Antibody - 100-401-J04S

Antibody type

Polyclonal

Vial size

25 µl

Western blot

Supportive validation

Submitted by

Rockland Immunochemicals, Inc. (provider)

Main image

Experimental details

Western Blot of Rabbit anti-Eg-5 Antibody. Lane 1: E.coli cell lysate expressing histidine tagged protein. Lane 2: Mouse brain lysate. Lane 3: Recombinant truncated Eg-5. Load: 35 µg per lane for cell lysate. 50 ng of recombinant protein Primary antibody: Eg-5 antibody at 1:1000 for overnight at 4°C. Secondary antibody: HRP rabbit secondary antibody at 1:40,000 for 60 min at RT. Block: 5% BLOTTO overnight at 4°C. Predicted/Observed size: 120 kDa and 72 kDa for Eg5.

Validation comment

Western Blot