NBP1-46196

antibody from Novus Biologicals

Targeting: GTF2E1 FE, TFIIE-A

Western blot (Supportive data in Antibodypedia)

Immunoprecipitation (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

NBP1-46196

Provider

Novus Biologicals

Proper citation

Novus Cat#NBP1-46196, RRID:AB_10009428

Product name

Rabbit Polyclonal TFIIE-alpha Antibody

Antibody type

Polyclonal

Description

Immunogen affinity purified.

Reactivity

Human, Mouse

Host

Rabbit

Isotype

IgG

Vial size

0.1 ml

Concentration

0.2 mg/ml

Storage

Store at 4C. Do not freeze.

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: TFIIE-alpha Antibody [NBP1-46196] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded), 293T (T; 50 ug), and mouse NIH3T3 (M; 50 ug) cells. Antibodies: Affinity purified rabbit anti-TFIIE-alpha antibody NBP1-46196 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. TFIIE-alpha was also immunoprecipitated by rabbit anti-TFIIE-alpha antibody NBP1-46195, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 3 seconds (B).

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: TFIIE-alpha Antibody [NBP1-46196] - Section of human colon carcinoma (left) and mouse renal cell carcinoma (right). Antibody: Affinity purified rabbit anti- GTF2E1/TFIIE-alpha used at a dilution of 1:200 (1ug/ml). Detection: DAB