MA5-38118

antibody from Invitrogen Antibodies

Targeting: PRPF8 hPrp8, Prp8, PRPC8, RP13, SNRNP220

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-38118

Provider

Invitrogen Antibodies

Product name

PRP8 Recombinant Rabbit Monoclonal Antibody (4I1Q8)

Antibody type

Monoclonal

Antigen

Recombinant full-length protein

Description

Positive Samples: HeLa, 293T, Jurkat, Mouse brain, Mouse spleen Immunogen sequence: MADNPSWDGE KTIIITCSFT PGSCTLTAYK LTPSGYEWGR QNTDKGNNPK GYLPSHYERV QMLLSDRFLG FFMVPAQSSW NYNFMGVRHD PNMKYELQLA NPKEFYHEVH RPSHFLNFAL LQEGEVYSAD REDLYA

Reactivity

Human, Mouse, Rat

Host

Rabbit

Isotype

IgG

Antibody clone number

4I1Q8

Vial size

100 µL

Concentration

1.2 mg/mL

Storage

-20°C, Avoid Freeze/Thaw Cycles

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of PRP8 in extracts of various cell lines. Samples were incubated with PRP8 Monoclonal antibody (Product # MA5-38118) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Immunocytochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunofluorescence analysis of PRP8 in U-2 OS cells. Samples were incubated with PRP8 Monoclonal antibody (Product # MA5-38118) using a dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence analysis of PRP8 in U-2 OS cells using PRP8 Recombinant Monoclonal Antibody (Product # MA5-38118) at a dilution of 1:100. Blue: DAPI for nuclear staining.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRP8 in paraffin-embedded rat brain. Samples were incubated with PRP8 Monoclonal antibody (Product # MA5-38118) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRP8 in paraffin-embedded human colon. Samples were incubated with PRP8 Monoclonal antibody (Product # MA5-38118) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRP8 in paraffin-embedded mouse spinal cord. Samples were incubated with PRP8 Monoclonal antibody (Product # MA5-38118) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRP8 in paraffin-embedded rat brain. Samples were incubated with PRP8 Monoclonal antibody (Product # MA5-38118) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRP8 in paraffin-embedded human colon. Samples were incubated with PRP8 Monoclonal antibody (Product # MA5-38118) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry analysis of PRP8 in paraffin-embedded mouse spinal cord. Samples were incubated with PRP8 Monoclonal antibody (Product # MA5-38118) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.