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Western blot
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Immunohistochemistry [1]
- Other assay [3]
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- Product number
- PA5-47034 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myostatin Polyclonal Antibody
- Antibody type
- Polyclonal
- Antigen
- Recombinant full-length protein
- Description
- In Western blots, approximately15% cross-reactivity is observed with recombinant human/mouse/rat GDF-11. In direct ELISAs, this antibody shows approximately 5% cross-reactivity with recombinant mouse (rm) GDF-5 and rmGDF-6. Reconstitute at 0.2 mg/mL in sterile PBS. Endoxin level is
- Reactivity
- Human, Mouse, Rat
- Host
- Goat
- Isotype
- IgG
- Vial size
- 100 μg
- Concentration
- 0.2 mg/mL
- Storage
- -20°C, Avoid Freeze/Thaw Cycles
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Immunohistochemical analysis of Myostatin in immersion fixed frozen sections of mouse embryo (10 d.p.c., section through neural tube). Samples were incubated in Myostatin polyclonal antibody (Product # PA5-47034) using a dilution of 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown) and counterstained with hematoxylin (blue).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of Myostatin in K562 human chronic myelogenous leukemia cell line. Samples were incubated in Myostatin polyclonal antibody (Product # PA5-47034). Recombinant MouseGDF-8/Myostatin increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF‚8/Myostatin Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.6-3 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization of Myostatin in K562 human chronic myelogenous leukemia cell line. Samples were incubated in Myostatin polyclonal antibody (Product # PA5-47034). Recombinant MouseGDF-8/Myostatin increases hemoglobin expression in the K562 human chronic myelogenous leukemia cell line in a dose-dependent manner (orange line), as measured by the psuedoperoxidase assay. Hemoglobin expression elicited by Recombinant Mouse GDF-8/Myostatin (30 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human/Mouse/Rat GDF‚8/Myostatin Antigen Affinity-purified Polyclonal Antibody. The ND50 is typically 0.6-3 µg/mL.
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Neutralization antibody testing demonstrates the specificity of an antibody through a correlation between antibody binding and the activity of the target. Neutralization of Myostatin is shown by the decrease in mean OD (measured by pseudoperoxidase assay) with increasing concentrations of Myostatin polyclonal antibody (Product # PA5-47034).
