MA5-15486

antibody from Invitrogen Antibodies

Targeting: MSTN GDF8

Western blot (Supportive data in Antibodypedia)

ELISA (Recommended by provider)

Other assay (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-15486

Provider

Invitrogen Antibodies

Product name

Myostatin Monoclonal Antibody (6H12)

Antibody type

Monoclonal

Antigen

Purifed from natural sources

Description

MA5-15486 targets Myostatin in indirect ELISA, WB applications and shows reactivity with Human samples. The MA5-15486 immunogen is purified recombinant fragment of Myostatin expressed in E. Coli. . MA5-15486 detects Myostatin which has a predicted molecular weight of approximately 42.7kDa.

Reactivity

Human

Host

Mouse

Isotype

IgG

Antibody clone number

6H12

Vial size

100 µL

Concentration

Conc. Not Determined

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

References

Submitted references

Overexpression of Latent TGFβ Binding Protein 4 in Muscle Ameliorates Muscular Dystrophy through Myostatin and TGFβ.

Lamar KM, Bogdanovich S, Gardner BB, Gao QQ, Miller T, Earley JU, Hadhazy M, Vo AH, Wren L, Molkentin JD, McNally EM
PLoS genetics 2016 May;12(5):e1006019

Western blot

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Western blot analysis of Myostatin using a Myostatin monoclonal antibody (Product # MA5-15486) against a truncated Myostatin-His recombinant protein (1).

Other assay

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Fig 4 LTBP4 interacts with myostatin, and mice overexpressing LTBP4 have increased strength and bigger muscle fibers. (A) 10 week old TG+ and WT mice were sacrificed and muscle lysates were obtained by combining quadriceps, gastrocnemius, soleus, gluteus, hamstring, and triceps muscles. Lysates were immunoprecipitated with an anti-myostatin followed by immunoblotting with anti-LTBP4 antibody, showing that LTBP4 associates with myostatin in vivo. (B) Forelimb grip strength of TG+ mdx (n = 8) and mdx (n = 8) mice was measured over ten trials, averaged, and normalized to tibial length. (kgf, kilogram force) Twenty week old TG+ mdx mice were significantly stronger than mdx controls (p = .011, Student's t-test). (C) Fiber area was determined by outlining hematoxylin and eosin stained quadriceps muscle fibers from TG+ mdx mice (n = 7) and mdx controls (n = 6) at 8 weeks of age. A histogram of fiber area shows that TG+ mdx mice had fewer small fibers and an increased number of large fibers. (D) TG+ mdx mice had significantly increased fiber area compared to mdx controls (p