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Western blotAntibody data
- Antibody Data
- Antigen structure
- References [1]
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- Validations
- Western blot [1]
- Other assay [1]
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- Product number
- MA5-15486 - Provider product page
- Provider
- Invitrogen Antibodies
- Product name
- Myostatin Monoclonal Antibody (6H12)
- Antibody type
- Monoclonal
- Antigen
- Purifed from natural sources
- Description
- MA5-15486 targets Myostatin in WB applications and shows reactivity with Human samples.
- Antibody clone number
- 6H12
- Concentration
- Conc. Not Determined
Submitted references Overexpression of Latent TGFβ Binding Protein 4 in Muscle Ameliorates Muscular Dystrophy through Myostatin and TGFβ.
Lamar KM, Bogdanovich S, Gardner BB, Gao QQ, Miller T, Earley JU, Hadhazy M, Vo AH, Wren L, Molkentin JD, McNally EM
PLoS genetics 2016 May;12(5):e1006019
PLoS genetics 2016 May;12(5):e1006019
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Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Western blot analysis of Myostatin using a Myostatin monoclonal antibody (Product # MA5-15486) against a truncated Myostatin-His recombinant protein (1).
Supportive validation
- Submitted by
- Invitrogen Antibodies (provider)
- Main image
- Experimental details
- Fig 4 LTBP4 interacts with myostatin, and mice overexpressing LTBP4 have increased strength and bigger muscle fibers. (A) 10 week old TG+ and WT mice were sacrificed and muscle lysates were obtained by combining quadriceps, gastrocnemius, soleus, gluteus, hamstring, and triceps muscles. Lysates were immunoprecipitated with an anti-myostatin followed by immunoblotting with anti-LTBP4 antibody, showing that LTBP4 associates with myostatin in vivo. (B) Forelimb grip strength of TG+ mdx (n = 8) and mdx (n = 8) mice was measured over ten trials, averaged, and normalized to tibial length. (kgf, kilogram force) Twenty week old TG+ mdx mice were significantly stronger than mdx controls (p = .011, Student's t-test). (C) Fiber area was determined by outlining hematoxylin and eosin stained quadriceps muscle fibers from TG+ mdx mice (n = 7) and mdx controls (n = 6) at 8 weeks of age. A histogram of fiber area shows that TG+ mdx mice had fewer small fibers and an increased number of large fibers. (D) TG+ mdx mice had significantly increased fiber area compared to mdx controls (p
