MA5-37712

antibody from Invitrogen Antibodies

Targeting: PGK1

Western blot (Data presented on provider website)

Immunohistochemistry (Supportive data in Antibodypedia)

Flow cytometry (Supportive data in Antibodypedia)

Antibody Data

Product number

MA5-37712

Provider

Invitrogen Antibodies

Product name

PGK1 Monoclonal Antibody (1086CT10.2.1)

Antibody type

Monoclonal

Antigen

Synthetic peptide

Reactivity

Human, Mouse

Host

Mouse

Isotype

IgG

Antibody clone number

1086CT10.2.1

Vial size

200 µL

Concentration

0.5 mg/mL

Storage

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Immunohistochemistry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PGK1 in human kidney tissue sections using the PGK1 Monoclonal Antibody (Product # MA5-37712) at a dilution of 1:25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Immunohistochemistry (Paraffin) analysis of PGK1 in human liver tissue sections using the PGK1 Monoclonal Antibody (Product # MA5-37712) at a dilution of 1:25. Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1:25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.

Flow cytometry

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis showing Jurkat cells stained with PGK1 Monoclonal Antibody (Product # MA5-37712) at a 1:25 dilution (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by the PGK1 Monoclonal Antibody for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.

Supportive validation

Submitted by

Invitrogen Antibodies (provider)

Main image

Experimental details

Flow Cytometry analysis showing Jurkat cells stained with PGK1 Monoclonal Antibody (Product # MA5-37712) at a 1:25 dilution (green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then incubated in 2% BSA to block non-specific protein-protein interactions followed by the PGK1 Monoclonal Antibody for 60 min at 37°C. The secondary antibody used was Goat-Anti-Mouse IgG, DyLight® 488 Conjugated Highly Cross-Adsorbed at 1:200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was mouse IgG2a (1µg/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.