654101

antibody from BioLegend

Targeting: TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B

Western blot (Supportive data in Antibodypedia)

Antibody Data

Product number

654101

Provider

BioLegend

Proper citation

BioLegend Cat#654101, RRID:AB_2561781

Product name

Purified anti-TIF1ß (KAP-1, TRIM28) Phospho (Ser473)

Antibody type

Monoclonal

Antigen

Modified peptide: VKRSRpSGEGEVC.

Reactivity

Human

Host

Mouse

Conjugate

Unconjugated

Isotype

IgG

Antibody clone number

11G10SC

Vial size

25 µg

Concentration

0.5 mg/ml

Storage

4° C

Handling

Ambient RT

Western blot

Supportive validation

Submitted by

BioLegend (provider)

Main image

Experimental details

Hela cell extracts untreated (lane 1) and treated with UV radiation (lane 2) were resolved by electrophoresis, transferred to nitrocellulose, and probed with anti-TIF1β phosphorylated (Ser473) antibody (clone 11G10SC). Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.

Conjugate

Unconjugated