Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunocytochemistry [1]
- Immunohistochemistry [1]
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Validation data
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- Product number
- NBP2-30022 - Provider product page
- Provider
- Novus Biologicals
- Product name
- Rabbit Polyclonal KAP1 Antibody
- Antibody type
- Polyclonal
- Description
- Protein A purified.
- Reactivity
- Human
- Host
- Rabbit
- Isotype
- IgG
- Vial size
- 0.4 ml
- Concentration
- 0.5 mg/ml
- Storage
- Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
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Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KAP1 Antibody [NBP2-30022] - western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the TRIM28 antibody detected the TRIM28 protein (arrow).
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Western Blot: KAP1 Antibody [NBP2-30022] - All lanes : Anti-TRIM28 Antibody (N-term) at 1:1000 dilution Lane 1: Hela whole cell lysate Lane 2: PC-3 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 89 kDa Blocking/Dilution buffer: 5% NFDM/TBST.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunocytochemistry/Immunofluorescence: KAP1 Antibody [NBP2-30022] - Fluorescent confocal image of Hela cell stained with TRIM28 Antibody (N-term)(NBP2-30022).Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TRIM28 primary antibody (1:25, 1 h at 37). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TRIM28 immunoreactivity is localized to Nucleus significantly.
Supportive validation
- Submitted by
- Novus Biologicals (provider)
- Main image
- Experimental details
- Immunohistochemistry-Paraffin: KAP1 Antibody [NBP2-30022] - immunohistochemistry analysis in formalin fixed and paraffin embedded human testis tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of TRIM28 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.