15202-1-AP

antibody from Proteintech Group

Targeting: TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B

Provider product page for 15202-1-AP

Western blot

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Immunocytochemistry

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Immunohistochemistry

Flow cytometry

Antibody data

Antibody Data
Antigen structure
References [32]
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Validations
Western blot [1]
Immunocytochemistry [1]
Immunohistochemistry [2]

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Product number: 15202-1-AP - Provider product page
Provider: Proteintech Group
Proper citation: Proteintech Cat#15202-1-AP, RRID:AB_2209890
Product name: KAP1 antibody
Antibody type: Polyclonal
Description: KD/KO validated KAP1 antibody (Cat. #15202-1-AP) is a rabbit polyclonal antibody that shows reactivity with human, mouse, rat and has been validated for the following applications: FC, IF, IHC, IP, WB, ELISA.
Reactivity: Human, Mouse, Rat
Host: Rabbit
Conjugate: Unconjugated
Isotype: IgG
Vial size: 20ul, 150ul

TRIM28 protein structure - 15202-1-AP shown in red.

Submitted references TRIM28 facilitates type I interferon activation by targeting TBK1.

Hua F, Nass T Jr, Parvatiyar K
Frontiers in immunology 2024;15:1279920

Retinoblastoma-associated protein is important for TRIM24-mediated activation of the mTOR signaling pathway through DUSP2 action in prostate cancer.

Ren D, Li W, Zeng R, Liu X, Liang H, Xiong W, Yang C, Jin X
Cell death and differentiation 2024 May;31(5):592-604

KAP1 stabilizes MYCN mRNA and promotes neuroblastoma tumorigenicity by protecting the RNA m(6)A reader YTHDC1 protein degradation.

Yang Y, Zhang Y, Chen G, Sun B, Luo F, Gao Y, Feng H, Li Y
Journal of experimental & clinical cancer research : CR 2024 May 14;43(1):141

Blocking the interaction between circTNRC18 and LIN28A promotes trophoblast epithelial-mesenchymal transformation and alleviates preeclampsia.

Chen LL, Li YQ, Kang ZH, Zhang X, Gu SY, Wang N, Shen XY
Molecular and cellular endocrinology 2024 Jan 1;579:112073

Cellular RNA and DNA sensing pathways are essential for the dose-dependent response of human monocytes to ionizing radiation.

Mikhalkevich N, Russ E, Iordanskiy S
Frontiers in immunology 2023;14:1235936

URI alleviates tyrosine kinase inhibitors-induced ferroptosis by reprogramming lipid metabolism in p53 wild-type liver cancers.

Ding Z, Pan Y, Shang T, Jiang T, Lin Y, Yang C, Pang S, Cui X, Wang Y, Feng XF, Xu M, Pei M, Chen Y, Li X, Ding J, Tan Y, Wang H, Dong L, Wang L
Nature communications 2023 Oct 7;14(1):6269

TRIM28 recruits E2F1 to regulate CBX8-mediated cell proliferation and tumor metastasis of ovarian cancer.

Zhang F, Zhu T, Wu C, Shen D, Liu L, Chen X, Guan Y, Ding H, Tong X
Human cell 2023 Nov;36(6):2113-2128

Trim28 citrullination maintains mouse embryonic stem cell pluripotency via regulating Nanog and Klf4 transcription.

Zhang Y, Wan X, Qiu L, Zhou L, Huang Q, Wei M, Liu X, Liu S, Zhang B, Han J
Science China. Life sciences 2023 Mar;66(3):545-562

ZNF133 is a potent suppressor in breast carcinogenesis through dampening L1CAM, a driver for tumor progression.

Li L, Wang X, Hu K, Liu X, Qiu L, Bai C, Cui Y, Wang B, Wang Z, Wang H, Cheng R, Hua J, Hai L, Wang M, Liu M, Song Z, Xiao C, Li B
Oncogene 2023 Jun;42(27):2166-2182

The Host E3-Ubiquitin Ligase TRIM28 Impedes Viral Protein GP4 Ubiquitination and Promotes PRRSV Replication.

Cui Z, Zhou L, Zhao S, Li W, Li J, Chen J, Zhang Y, Xia P
International journal of molecular sciences 2023 Jun 30;24(13)

Nucleoporin Seh1 maintains Schwann cell homeostasis by regulating genome stability and necroptosis.

Wu M, Li M, Liu W, Yan M, Li L, Ding W, Nian X, Dai W, Sun D, Zhu Y, Huang Q, Lu X, Cai Z, Hong F, Li X, Zhang L, Liu Z, Mo W, Zhang X, Zhang L
Cell reports 2023 Jul 25;42(7):112802

Cancer associated fibroblast derived SLIT2 drives gastric cancer cell metastasis by activating NEK9.

Lu G, Du R, Dong J, Sun Y, Zhou F, Feng F, Feng B, Han Y, Shang Y
Cell death & disease 2023 Jul 13;14(7):421

TRIM24 controls induction of latent HIV-1 by stimulating transcriptional elongation.

Horvath RM, Dahabieh M, Malcolm T, Sadowski I
Communications biology 2023 Jan 23;6(1):86

Alternative transcribed 3' isoform of long non-coding RNA Malat1 inhibits mouse retinal oxidative stress.

Ghanam AR, Ke S, Wang S, Elgendy R, Xie C, Wang S, Zhang R, Wei M, Liu W, Cao J, Zhang Y, Zhang Z, Xue T, Zheng Y, Song X
iScience 2023 Jan 20;26(1):105740

POGZ suppresses 2C transcriptional program and retrotransposable elements.

Sun X, Zhang T, Tong B, Cheng L, Jiang W, Sun Y
Cell reports 2023 Aug 29;42(8):112867

RNAPII-dependent ATM signaling at collisions with replication forks.

Einig E, Jin C, Andrioletti V, Macek B, Popov N
Nature communications 2023 Aug 24;14(1):5147

A method to map the interaction network of the nuclear lamina with genetically encoded photo-crosslinkers in vivo.

Neumann-Staubitz P, Kitsberg D, Buxboim A, Neumann H
Frontiers in chemistry 2022;10:905794

Retinitis pigmentosa 2 pathogenic mutants degrade through BAG6/HUWE1 complex.

Zhang J, Gao H, Jiang N, Jing M, Sun Z, Du C, Zhang J, Wang M, Li J, Gao F, Hu Y, Mu H, Cui X
Experimental eye research 2022 Jul;220:109110

Smoking-associated upregulation of CBX3 suppresses ARHGAP24 expression to activate Rac1 signaling and promote tumor progression in lung adenocarcinoma.

Jin X, Zhang B, Zhang H, Yu H
Oncogene 2022 Jan;41(4):538-549

WASH interacts with Ku to regulate DNA double-stranded break repair.

Wang T, Du XH, Hong Y, Hong X, Fan L, Zhou JW, Sun H, Ge J, Billadeau DD, Deng ZH
iScience 2022 Jan 21;25(1):103676

Phosphorylation of the HP1β hinge region sequesters KAP1 in heterochromatin and promotes the exit from naïve pluripotency.

Qin W, Ugur E, Mulholland CB, Bultmann S, Solovei I, Modic M, Smets M, Wierer M, Forné I, Imhof A, Cardoso MC, Leonhardt H
Nucleic acids research 2021 Jul 21;49(13):7406-7423

ZNF382 controls mouse neuropathic pain via silencer-based epigenetic inhibition of Cxcl13 in DRG neurons.

Ma L, Yu L, Jiang BC, Wang J, Guo X, Huang Y, Ren J, Sun N, Gao DS, Ding H, Lu J, Zhou H, Zou L, Gao Y, Wang L, Sun K, Ming Y, Meng Z, Tao YX, Yan M
The Journal of experimental medicine 2021 Dec 6;218(12)

TRIM28 maintains genome imprints and regulates development of porcine SCNT embryos.

Zhai Y, Zhang M, An X, Zhang S, Kong X, Li Q, Yu H, Dai X, Li Z
Reproduction (Cambridge, England) 2021 Apr;161(4):411-424

TRIM28 Expression on Dendritic Cells Prevents Excessive T Cell Priming by Silencing Endogenous Retrovirus.

Chikuma S, Yamanaka S, Nakagawa S, Ueda MT, Hayabuchi H, Tokifuji Y, Kanayama M, Okamura T, Arase H, Yoshimura A
Journal of immunology (Baltimore, Md. : 1950) 2021 Apr 1;206(7):1528-1539

TRIM28 functions as the SUMO E3 ligase for PCNA in prevention of transcription induced DNA breaks.

Li M, Xu X, Chang CW, Liu Y
Proceedings of the National Academy of Sciences of the United States of America 2020 Sep 22;117(38):23588-23596

TRIM28 protects CARM1 from proteasome-mediated degradation to prevent colorectal cancer metastasis.

Cui J, Hu J, Ye Z, Fan Y, Li Y, Wang G, Wang L, Wang Z
Science bulletin 2019 Jul 30;64(14):986-997

TRIM28 promotes HIV-1 latency by SUMOylating CDK9 and inhibiting P-TEFb.

Ma X, Yang T, Luo Y, Wu L, Jiang Y, Song Z, Pan T, Liu B, Liu G, Liu J, Yu F, He Z, Zhang W, Yang J, Liang L, Guan Y, Zhang X, Li L, Cai W, Tang X, Gao S, Deng K, Zhang H
eLife 2019 Jan 17;8

TRIM28 promotes cervical cancer growth through the mTOR signaling pathway.

Li F, Wang Z, Lu G
Oncology reports 2018 Apr;39(4):1860-1866

ASCIZ/ATMIN is dispensable for ATM signaling in response to replication stress.

Liu R, King A, Hoch NC, Chang C, Kelly GL, Deans AJ, Heierhorst J
DNA repair 2017 Sep;57:29-34

TRIM28 knockdown increases sensitivity to etoposide by upregulating E2F1 in non-small cell lung cancer.

Liu L, Xiao L, Liang X, Chen L, Cheng L, Zhang L, Wu X, Xu Q, Ma C
Oncology reports 2017 Jun;37(6):3597-3605

TRIM28, a new molecular marker predicting metastasis and survival in early-stage non-small cell lung cancer.

Liu L, Zhao E, Li C, Huang L, Xiao L, Cheng L, Huang X, Song Y, Xu D
Cancer epidemiology 2013 Feb;37(1):71-8

Liver-specific ablation of Krüppel-associated box-associated protein 1 in mice leads to male-predominant hepatosteatosis and development of liver adenoma.

Bojkowska K, Aloisio F, Cassano M, Kapopoulou A, Santoni de Sio F, Zangger N, Offner S, Cartoni C, Thomas C, Quenneville S, Johnsson K, Trono D
Hepatology (Baltimore, Md.) 2012 Oct;56(4):1279-90

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Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: HeLa cells were subjected to SDS PAGE followed by western blot with 15202-1-AP(KAP1 antibody) at dilution of 1:3000
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunofluorescent analysis of Hela cells, using TRIM28 antibody 15202-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).
Sample type: cell line

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 15202-1-AP(KAP1 antibody) at dilution of 1:100 (under 10x lens)
Sample type: tissue

Supportive validation

Submitted by: Proteintech Group (provider)
Main image
Experimental details: Immunohistochemical of paraffin-embedded human colon cancer using 15202-1-AP(KAP1 antibody) at dilution of 1:100 (under 40x lens)
Sample type: tissue