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Western blot
Immunoprecipitation
ImmunohistochemistryAntibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Western blot [2]
- Immunohistochemistry [6]
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- Product number
- LS-C287178 - Provider product page
- Provider
- LSBio
- Product name
- TRIM28 / KAP1 Antibody (aa650-700) LS-C287178
- Antibody type
- Polyclonal
- Description
- Affinity purified
- Reactivity
- Human, Mouse
- Host
- Rabbit
- Isotype
- IgG
- Storage
- Store at 2-8°C for up to one year.
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Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human and Mouse KAP-1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 ug), 293T and mouse TCMK-1 cells. Antibodies: Affinity purified rabbit anti-KAP-1 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human and mouse KAP-1 by western blot. Samples: Whole cell lysate (50 µg) from HeLa, HEK293T, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-KAP-1 antibody used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 1 second.
Supportive validation
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human KAP-1 by Immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and ovarian carcinoma (right). Antibody: Affinity purified rabbit anti-KAP-1 used at a dilution of 1:5000 (0.2and 1:1000 (1 Detection: DAB.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of mouse Kap-1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytomaAntibody: Affinity purified rabbit anti-Kap-1 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human Kap-1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of Human KAP-1 by Immunohistochemistry. Sample: FFPE sections of human lung carcinoma (left) and ovarian carcinoma (right). Antibody: Affinity purified rabbit anti-KAP-1 used at a dilution of 1:5000 (0.2and 1:1000 (1 Detection: DAB.
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of mouse Kap-1 by immunohistochemistry. Sample: FFPE section of mouse plasmacytomaAntibody: Affinity purified rabbit anti-Kap-1 used at a dilution of 1:1,000 (1µg/ml). Detection: DAB
- Submitted by
- LSBio (provider)
- Enhanced method
- Genetic validation
- Main image
- Experimental details
- Detection of human Kap-1 by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Kap-1 used at a dilution of 1:5,000 (0.2µg/ml). Detection: DAB
