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Western blotAntibody data
- Antibody Data
- Antigen structure
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- Validations
- Western blot [1]
- Immunocytochemistry [1]
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- Product number
- TA328012 - Provider product page
- Provider
- OriGene
- Product name
- Mouse Monoclonal anti-TIF1¦Â Antibody
- Antibody type
- Monoclonal
- Description
- Mouse Monoclonal anti-TIF1¦Â Antibody
- Host
- Mouse
- Conjugate
- Unconjugated
- Epitope
- TRIM28
- Isotype
- IgG
- Antibody clone number
- 20A1
- Vial size
- 100 µg
- Concentration
- 0.5 mg/ml
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Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HepG2 nuclear extract was resolved by electrophoresis, transferred to nitrocellulose and probed with monoclonal anti-TIF1ß antibody. Proteins were visualized using a goat anti-mouse secondary conjugated to HRP and a chemiluminescence detection system.
- Validation comment
- WB
Supportive validation
- Submitted by
- OriGene (provider)
- Main image
- Experimental details
- HeLa cells were intracellularly stained with 5 ug/ml of purified anti-TIF1β (KAP-1, TRIM28) (clone 20A1) in blocking buffer overnight at 4?, followed by DyLight™ 594 anti-mouse IgG (red) and Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DAPI (blue). The image was captured with 20X objective.
- Validation comment
- IF
