Antibody data
- Antibody Data
- Antigen structure
- References [0]
- Comments [0]
- Validations
- Immunoelectron microscopy [1]
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Validation data
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- Product number
- 619310 - Provider product page
- Provider
- BioLegend
- Proper citation
- BioLegend Cat#619310, RRID:AB_2616773
- Product name
- Brilliant Violet 421™ anti-TIF1β (KAP-1, TRIM28)
- Antibody type
- Monoclonal
- Antigen
- Other
- Reactivity
- Human
- Host
- Mouse
- Conjugate
- Blue dye
- Antibody clone number
- 20A1
- Vial size
- 50 µg
- Concentration
- 0.2 mg/ml
- Storage
- 2°C-8°C
- Handling
- Ambient RT
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Supportive validation
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- BioLegend (provider)
- Main image
- Experimental details
- HeLa cells were fixed with 1% paraformaldehyde (PFA) for ten minutes, permeabilized with 0.5% Triton X-100 for ten minutes, and blocked with 5% FBS for 30 minutes. Then, the cells were intracellularly stained with 5 µg/ml of anti-TIF1β (clone 20A1) Brilliant Violet 421™ (blue) in 5% FBS overnight at 4°C, followed by Alexa Fluor® 488 Phalloidin (green) staining for 20 minutes. Nuclei were counterstained with DRAQ7™ (red). The image was captured with 40X objective.
- Conjugate
- Blue dye