720016

antibody from Invitrogen Antibodies

Targeting: TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B

Western blot

Antibody data

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Validation data

Product number: 720016 - Provider product page
Provider: Invitrogen Antibodies
Product name: TRIM28 Polyclonal Antibody
Antibody type: Polyclonal
Antigen: Synthetic peptide
Description: These Polyclonal antibodies are of rabbit origin developed by immunizing animals with proteins or peptides. The polyclonal antibody is purified by affinity purification from the rabbit sera generated after immunizing the rabbits with a specific type of protein or peptide. The purified antibody is tested for its functionality in various relevant research applications. The antibody is developed for Research Use Only and is non-hazardous or non-infectious in nature. This antibody is predicted to react with Monkey, Rat and Mouse.
Reactivity: Human
Host: Rabbit
Isotype: IgG
Vial size: 100 µg
Concentration: 0.5 mg/mL
Storage: Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

TRIM28 protein structure - 720016 shown in red.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: For immunofluorescence analysis, etoposide (25 uM, 6 h) treated HeLa cells were fixed and permeabilized for detection of TIF1 beta using TIF1 beta Rabbit Polyclonal Antibody (Product # 720016, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TIF1 beta protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TIF1 beta. Panel e) shows untreated cells with no signal. Panel f) represents control cells with no primary antibody to assess background.

Supportive validation

Submitted by: Invitrogen Antibodies (provider)
Main image
Experimental details: For immunofluorescence analysis, etoposide (25 uM, 6 h) treated HeLa cells were fixed and permeabilized for detection of TIF1 beta using TIF1 beta Rabbit Polyclonal Antibody (Product # 720016, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of TIF1 beta protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). Panel c) represents cytoskeletal F-actin staining using Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d) is a composite image of Panels a, b and c clearly demonstrating nuclear localization of TIF1 beta. Panel e) shows untreated cells with no signal. Panel f) represents control cells with no primary antibody to assess background.