H00010155-M01

antibody from Novus Biologicals

Targeting: TRIM28 KAP1, PPP1R157, RNF96, TF1B, TIF1B

Western blot (Supportive data in Antibodypedia)

ELISA (Data presented on provider website)

Immunocytochemistry (Supportive data in Antibodypedia)

Immunohistochemistry (Supportive data in Antibodypedia)

Blocking/Neutralizing (Data presented on provider website)

Antibody Data

Product number

H00010155-M01

Provider

Novus Biologicals

Proper citation

Novus Cat#H00010155-M01, RRID:AB_829308

Product name

Mouse Monoclonal KAP1 Antibody

Antibody type

Monoclonal

Description

IgG purified. TRIM28 (4E6)

Reactivity

Human, Mouse, Rat

Host

Mouse

Isotype

IgG

Vial size

0.1 mg

Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

References

Submitted references

Multipotent adult germline stem cells and embryonic stem cells functional proteomics revealed an important role of eukaryotic initiation factor 5A (Eif5a) in stem cell differentiation.

Dihazi H, Dihazi GH, Jahn O, Meyer S, Nolte J, Asif AR, Mueller GA, Engel W
Journal of proteome research 2011 Apr 1;10(4):1962-73

Western blot

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi ( Cat # H00010155-R01V ) (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody (M01) clone 4E6 (Cat # H00010155-M01 ). GAPDH ( 36.1 kDa ) used as specificity and loading control.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M01), clone 4E6.Lane 1: TRIM28 transfected lysate(88.5 KDa).Lane 2: Non-transfected lysate.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6 Analysis of TRIM28 expression in Hela S3 NE.

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Western Blot: KAP1 Antibody (4E6) [H00010155-M01] - TRIM28 monoclonal antibody (M01), clone 4E6. Analysis of TRIM28 expression in PC-12.

Immunocytochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunocytochemistry/Immunofluorescence: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on HeLa cell. Antibody concentration 10 ug/ml.

Immunohistochemistry

Supportive validation

Submitted by

Novus Biologicals (provider)

Main image

Experimental details

Immunohistochemistry-Paraffin: KAP1 Antibody (4E6) [H00010155-M01] - Analysis of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. Antibody concentration 3 ug/ml.